Tagging of SSR markers associated to yellow mosaic virus resistance in black gram

In black gram, yellow mosaic virus (YMV) disease is a serious problem causing severe yield reduction. In the present study, F.sub.2 and RIL Population (F.sub.4:5) of the cross IC436656 (YMV susceptible) x KKB14045 (YMV resistant) were evaluated for YMV resistance through bulked segregant analysis (BSA) using SSR markers. Out of 91 SSR markers studied, thirteen were polymorphic between IC436656 and KKB14045 which showed 14.3% of genome diversity. The F.sub.2 population segregated as 3:1 for susceptibility and resistance in chi-square test which indicated monogenic recessive gene control the YMV resistance. From single marker analysis (SMA), CEDG141 and CEDG008 were strongly associated to YMV resistance. From BSA, CEDG141 differentiated IC436656 (200 bp) and KKB14045 (280 bp) in F.sub.2 population. CEDG141, CEDG 264 and CEDG 008 differentiated the RILs of the same population in BSA. The identified SSR markers would be valuable in black gram breeding programs for YMV resistance studies.