Synthesis of H-Lys-Lys-Gly-OH Tripeptide and Study of Its Acute Toxicity

Tripeptide H-Lys-Lys-Gly-OH was synthesized. Its toxic properties and biological activity were studied. The tripeptide was synthesized by extending the peptide chain from the C-terminus by synthesizing N 2 , N 6 -dicarbobenzoxylysine [ N 2 , N 6 -(Cbz) 2 Lys] and N 6 -carbobenzoxylysine ( N 6 -CbzLys) methyl ester hydrochloride and condensing them by the mixed anhydride method to produce the methyl ester of N 2 , N 6 -(Cbz) 2 Lys- N 6 -CbzLys. The next step in the tripeptide synthesis consisted of obtaining glycine benzyl ester (Gly-OBzl) hydrochloride and condensing it with N 2 , N 6 -(Cbz) 2 Lys- N 6 -CbzLys hydrazide to produce the protected target tripeptide Z -Lys( Z )-Lys-Gly-OBzl. The last step consisted of removing the protection using hydrogenolysis over Ni-Re catalyst. Test results showed that the synthesized tripeptide had low toxicity.