Image Analysis of Oxidative and Glycolytic Muscle Fibers During Reperfusion Injury by Segmentation Based on Regions/ Analisis de Imagenes de Fibras Musculares Oxidativas y Glucoliticas Durante la Reperfusion Mediante Segmentacion Basada en Regiones

Different situations cause ischemia and reperfusion injury, affecting tissues under the level of compression. In this research, abnormal characteristics in distribution of muscle fibers types in soleus and extensor carpi radialis longus, during short periods of ischemia and short and long periods of reperfusion, were determined. Fibers were classified by enzyme histochemistry techniques NADH-TR and myosin-ATPase. Measurements of areas were carried out through semiautomatic image processing by using segmentation based on regions, which evidenced significant changes in distribution during reperfusion followed to one and three hours of ischemia, as well as in comparisons of areas for all periods of reperfusion. This study strengthens the evidence about using practical procedures of image analysis in the diagnosis of tisular abnormalities. KEY WORDS: Histochemistry; Ischemia; Reperfusion; Image analysis; Segmentation. Diferentes situaciones causan isquemia y reperfusion afectando los tejidos que se encuentran por debajo del nivel de lesion. En esta investigacion, se determinaron las caracteristicas anormales en la distribucion de los tipos de fibras musculares en los musculos soleo y extensor radial longo del carpo, durante periodos cortos de isquemia y de reperfusion tanto cortos como prolongados. Las fibras se clasificaron mediante las tecnicas de tincion de histoquimica enzimatica NADH-TR y miosina-ATPasa. Las mediciones de las areas realizadas mediante procesamiento de imagenes y el uso de la segmentacion basada en regiones, evidenciaron cambios significativos en la distribucion durante la reperfusion seguida a una y tres horas de isquemia, asi como en las comparaciones para todos los periodos de reperfusion. Este estudio es un aporte en la evidencia sobre el uso de procedimientos practicos de analisis de imagenes en el diagnostico de alteraciones tisulares. PALABRAS CLAVE: Histoquimica; Isquemia; Reperfusion; Analisis de imagenes; Segmentacion.