Localization of Vesicular Inhibitory Amino Acid Transporter en la Glandula Salival Submandibular Durante el Desarrollo Postnatal de Ratones

According to recent studies, it is highly possible that the occurrence of vesicular inhibitory amino acid transporter (VIAAT) is a good marker of GABA-signaling not only in the brain, but also in extra-brain tissue cells containing GABA and GAD. In view of this, the present study was attempted to localize VIAAT-immunoreactivity in the submandibular gland of mice. In the present study, the submandibular glands of male mice at various postnatal developmental stages were examined for detailed localization of VIAAT-immunoreactivity in immunohistochemistry at light microscopic level. The immunoreactivity for VIAAT was localized in epithelial cells of proximal and distal excretory ducts with the striated portion more intensely immunopositive at young postnatal stages. No significant immunoreactivity was seen in the acinar cells throughout the postnatal development. In addition, the immunoreactivity for VIAAT was detected in the salivary parasympathetic ganglionic neurons, but not in any nerve fibers surrounding the glandular cells. Furthermore, VIAAT-immunoreactivity was found in smooth muscle cells forming the outermost layer of intralobular arterioles. From the present findings, it is possible that GABA plays roles as paracrine and autocrine regulators in the saliva secretion as well as the gland development. KEY WORDS: VIATT-immunoreactivity; GABA; Salivary glands; Arteriolar smooth muscle. Segun estudios recientes, es altamente posible que la aparicion del transportador vesicular de aminoacidos inhibidores (VIAAT) sea un buen marcador de senalizacion de GABA no solo en el cerebro, sino tambien en celulas de tejido extra-cerebrales que contienen GABA y GAD. En el presente estudio se intento localizar inmunoreactividad a VIAAT en la glandula submandibular de ratones. En el presente estudio, se examinaron las glandulas submandibulares de ratones machos en las distintas etapas del desarrollo postnatal para la localizacion detallada de inmunoreactividad a VIAAT inmunohistoquimicamente a nivel de microscopia optica. La inmunorreactividad para VIAAT se localizo en las celulas epiteliales de los conductos excretores proximal y distal, con mayor intensidad en la porcion estriada en las etapas tempranas. No se observo inmunoreactividad significativa en las celulas acinares durante el desarrollo postnatal. Ademas, se detecto la inmunoreactividad para VIAAT en las neuronas ganglionares parasimpaticas salivales, pero no en las fibras nerviosas que rodean las celulas glan