Brewery waste reuse for protease production by lactic acid fermentation

This study evaluated the use of three solid brewery wastes: brewer's spent grain, hot trub and residual brewer's yeast, as alternative media for the cultivation of lactic acid bacteria to evaluate their potential for proteolytic enzyme production. Initially, a mixture experimental design w...

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Veröffentlicht in:Food technology and biotechnology 2017-04, Vol.55 (2), p.218
Hauptverfasser: Mathias, Thiago Rocha dos Santos, de Aguiar, Paula Fernandes, de Almeida e Silva, Joao Batista, de Mello, Pedro Paulo Moretzsohn, Servulo, Eliana Flavia Camporese
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Sprache:eng
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Zusammenfassung:This study evaluated the use of three solid brewery wastes: brewer's spent grain, hot trub and residual brewer's yeast, as alternative media for the cultivation of lactic acid bacteria to evaluate their potential for proteolytic enzyme production. Initially, a mixture experimental design was used to evaluate the effect of each residue, as well as different mixtures (with the protein content set at 4%) in the enzyme production. At predetermined intervals, the solid and liquid fractions were separated and the extracellular proteolytic activity was determined. After selecting the best experimental conditions, a second experiment, factorial experimental design, was developed in order to evaluate the protein content in the media (1 to 7%) and the addition of fermentable sugar (glucose, 1 to 7%). Among the wastes, residual yeast showed the highest potential for the production of extracellular enzymes, generating a proteolytic extract with 2.6 U/mL in 3 h. However, due to the low content of the fermentable sugars in the medium, the addition of glucose also had a positive effect, increasing the proteolytic activity to 4.9 U/mL. The best experimental conditions of each experimental design were reproduced for comparison, and the enzyme content was separated by ethanol precipitation. The best medium produced a precipitated protein with proteolytic activity of 145.5 U/g. Key words: brewery waste, waste reuse, lactic fermentation, proteolytic enzymes
ISSN:1330-9862
DOI:10.1711/3ftb.55.02.17.4378