Nutrigenomic evaluation of garlic leaf powder supplementation on growth performance and immune characteristics in broilers

Aim: In this study, a planned research work was conducted to investigate the nutrigenomic aspects of supplementation of Allium sativum (garlic) and Ocimum sanctum (holy basil) leaf powder on the growth performance and immune characteristics of broilers. Materials and Methods: A 6 weeks feeding trial was conducted with 280-day-old Ven Cobb broilers, distributed randomly into seven experimental groups. Each treatment had 4 replicates with 10 birds each. The birds of the control group ([T.sub.1]) were fed a basal diet formulated as per BIS standards. The broilers of treatment groups [T.sub.2] and [T.sub.3] were fed basal diet supplemented with the commercially available garlic powder (GP) at levels of 0.5% and 1.0% of the feed, respectively, while broilers in [T.sub.4] and [T.sub.5] were fed basal diet supplemented with commercial grade holy basil leaf powder (HBLP) at levels 0.5% and 1.0% of the feed, respectively. Birds in the [T.sub.6] were fed with 0.5% GP and 0.5% HBLP, whereas [T.sub.7] was fed with 1.0% GP and 1.0% HBLP. At the end of the feeding trial (6th week), blood samples were collected and analyzed for relative mRNA expression of toll-like receptors (TLR) 2, TLR 4 and TLR 7 using real-time polymerase chain reaction. Results: The mean body weight gain and feed conversion efficiency were improved (p