Anatomical and cytometric characterization in biribazeiro

The biribazeiro (Rollinia mucosa [Jacq.]) is a native fruit of Central America and South America that stands out from the other species of the genus because it has large and edible fruits. Considering that little is known about the anatomy of the plant, the type of reserve of the seed, as well as little is known about the DNA content of species of Annonaceae family, this study aimed to characterize seeds histochemically and seedlings anatomically, and determine DNA content of biribazeiro (Rollinia mucosa [Jacq.]). The seedlings were obtained from seeds collected in the Amazon region by Embrapa Roraima and then, sent to UFLA, Federal University of Lavras, Minas Gerais State. The pulp of the fruits were extracted and the seeds were previously washed and sown in trays of 48 cells containing sawdust as substrate, remaining in a germination chamber at 30°C for 90 days. A histochemical analysis, anatomical characterization and determination of DNA content of the plants obtained were made. The main conclusions of this research were: (1) the seeds of Biribazeiro (Rollinia mucosa) present mainly lipid reserve, (2) The cross-sections of the leaf blade indicate dorsiventral organization, hypostomatic with paracitic stomata and trichomes on both sides of the sheet (3) the leaves of biribazeiro have on average 4.77 pg of DNA. Index terms: Anonaceae, anatomy, histochemistry, flow cytometry. O biribazeiro (Rollinia mucosa [Jacq.]) e uma frutifera nativa da America Central e America do Sul que se destaca das demais especies do genero por apresentar frutos grandes e comestiveis. Tendo em vista que pouco se conhece a respeito da anatomia da planta, tipo de reserva da semente, como tambem sobre o conteudo de DNA de especies da familia anonaceas, este trabalho teve por objetivos caracterizar histoquimicamente as sementes e anatomicamente as plantulas, e determinar o conteudo de DNA de biribazeiro. As plantulas foram obtidas de sementes coletadas na regiao Amazonica pela Embrapa Roraima e enviadas a Universidade Federal de Lavras. Os frutos foram despolpados, e suas sementes foram previamente lavadas e semeadas em bandejas de 48 celulas, contendo como substrato po de serragem, permanecendo em camara de germinacao a 30°C por 90 dias. Nas plantas obtidas, procedeu-se a analise histoquimica, a caracterizacao anatomica e a determinacao do conteudo de DNA. As principais conclusoes deste trabalho foram que: (1) as sementes da Rollinia mucosa apresentam reserva principal lipidica; (2