Encapsulation, dehydration and cryoprotection in regenerative capacity of shoot apices of Genipa americana/Encapsulamento, crioprotecao e desidratacao na capacidade regenerativa de apices caulinares de Genipa americana

Genipa americana (genipap) is a forest species belonging to the Rubiaceae family that has economic and environmental importance, being valued for food production, recuperation of degraded areas, in the composition in areas of permanent preservation and agroforestry. The objective of this research was to evaluate the effect of different times of dehydration in laminar flow cab and immersion time in cryoprotecting solution (MS+0.5M sucrose) on the regenerative capacity of shoot apices for establishing future cryopreservation protocols. The shoot apices were obtained from seedlings, of Oiteiros accession, germinated and cultured in vitro. Explants were subjected to encapsulation and different exposure times in cryoprotecting solution and dehydration in a laminar flow cab. The cryoprotection and dehydration does not modify the viability of the shoot apices of genipap encapsulated or unencapsulated. Immersion for 24 hours in cryoprotecting solution and the dehydration in a laminar flow cab by 2 hours have potential for use in future studies of cryopreservation by encapsulation-dehydration. Key words: genipap, Genipa americana, Conservation ex situ, Cryoprotectant. Genipa americana (jenipapeiro) e uma essencia florestal pertencente a familia Rubiaceae, que apresenta importancia economica e ambiental, sendo valorizada para producao de alimentos, na recuperacao de areas degradadas, na composicao em areas de preservacao permanentes e em sistemas agroflorestais. Objetivou-se avaliar o efeito de diferentes tempos de desidratacao em camara de fluxo laminar e tempos de imersao em solucao crioprotetora (MS+0,5M de sacarose) na capacidade regenerativa de apices caulinares da especie para estabelecimento de futuros protocolos de criopreservacao. Os apices caulinares foram obtidos de plantulas, acesso Oiteiros, germinadas e cultivadas in vitro. Os explantes foram submetidos ao encapsulamento e a diferentes tempos de imersao em solucao crioprotetora e tempos de desidratacao em camara de fluxo laminar. A crioprotecao e a desidratacao nao alteram a viabilidade dos apices caulinares de jenipapeiro encapsulados ou nao encapsulados. A imersao por 24 horas em solucao crioprotetora e a desidratacao em camara de fluxo laminar por 2 horas apresentam potencial para uso em futuros trabalhos de criopreservacao por encapsulamentodesidratacao. Palavras-chave: jenipapeiro, Genipa americana, Conservacao ex situ, Crioprotetor.