Serum and peritoneal protein profile of healthy horses and horses subjected to experimental intestinal obstruction/ Proteinograma serico e do liquido peritoneal de equinos higidos e daqueles submetidos a obstrucao intestinal experimental

It was evaluated the acute phase response over the concentration of acute phase proteins (APP) in serum and in peritoneal fluid of twenty-one healthy equines submitted to experimental intestinal obstruction distributed in groups: duodenal obstruction (GD), ileum obstruction (GI), left-dorsal colon (GM), and instrumentalized control (GC). Blood and peritoneal liquid samples were collected, after centrifugation and fractioning and the acute phase proteins were separated by electrophoresis in SDS-PAGE. The acute phase proteins (APP) identified were IgA, ceruloplasmin, transferrin, albumin, IgG, haptoglobin, a 1-acid glycoprotein and P24, in the serum and liquid peritoneal. There was anincrease in the serum and peritoneal proteins concentrations evidencing a higher response of the APP from the peritoneal fluid and of the obstructed groups. The electrophoretic separation of the proteins included in the peritoneal liquid is more effective when compared to the serum concentration in the diagnosis of abdominal inflammatory processes. Key words: blood, peritoneal fluid, horses, colic, acute phase proteins. Avaliou-se a resposta de fase aguda atraves da concentracao das proteinas de fase aguda (PFA) no soro sanguineo e no liquido peritoneal de vinte e um equinos, higidos e submetidos a obstrucao intestinal experimental, distribuidos em quatro grupos: obstrucao de duodeno - GD (n=6), ileo - GI (n=6), colon dorsal esquerdo - GM (n=6) e controle instrumentado - GC (n=3). Foram colhidas amostras de sangue e liquido peritoneal e, apos centrifugacao e fracionamento, as proteinas de fase aguda foram separadas por eletroforese em SDS-PAGE. Identificaramse as proteinas IgA, ceruloplasmina, transferrina, albumina, IgG, haptoglobina, a-glicoproteina acida e P24, no soro e no liquido peritoneal. Houve aumento nas concentracoes serica e peritoneal de todas as PFA, sendo mais evidente no liquido peritoneal e nos animais obstruidos. O fracionamento eletroforetico das PFA no liquido peritoneal e mais eficaz no diagnostico de processos inflamatorios abdominais, quando comparado ao serico. Palavras-chave: sangue, liquido peritoneal, equinos, colica, proteinas de fase aguda.