Potato somaclones identified by minimum descriptors sprout/Somaclones em batata identificados pelos descritores minimos de broto
It was examined the phenotypic stability of potato cultivars 'Asterix' and 'Macaca', evaluated the effect of explant type (direct and indirect organogenesis) and subculture time (12 and 70 months) in MS nutrient medium on the occurrence of somaclonal variation in both cultivars i...
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Veröffentlicht in: | Ciência rural 2015-08, Vol.45 (8), p.1381 |
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Hauptverfasser: | , , |
Format: | Artikel |
Sprache: | por |
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Zusammenfassung: | It was examined the phenotypic stability of potato cultivars 'Asterix' and 'Macaca', evaluated the effect of explant type (direct and indirect organogenesis) and subculture time (12 and 70 months) in MS nutrient medium on the occurrence of somaclonal variation in both cultivars in seed potato production through the use of seven minimum descriptors sprout. In 'Asterix' and 'Macaca' somaclones have occurred in four of the seven descriptors, however, only in the shape and in the base of the bud pubescence that somaclonal variation occurred simultaneously in both cultivars. Both genotypes are susceptible to the occurrence of somaclonal variation. It was identify the occurrence of somaclones both at 12 months and 70 months of subculture in both genotypes. Unlike the widely recorded, somaclones were identified in shoot apical segments and nodal segments derived from direct organogenesis in 'Asterix' and 'Macaca'. Key words: somaclonal variation, phenotipic stability, direct organogenesis, number of subcultures. Este estudo objetivou verificar a estabilidade fenotipica das cultivares de batata 'Asterix' e '.Macaca', avaliar o efeito do tipo de explante (organogenese direta e indireta) e do tempo de subcultivo (12 e 70 meses) em meio nutritivo MS sobre a ocorrencia de somaclones nas duas cultivares na producao de batata semente, mediante o emprego de sete descritores minimos de broto. Em 'Asterix' e '.Macaca' ocorreram somaclones em quatro dos sete descritores, contudo, apenas no formato e pubescencia da base do broto houve variacao, simultaneamente, em ambas. Os dois genotipos sao suscetiveis a ocorrencia de variacao somaclonal. Registrou-se somaclonesnos dois tempos de subcultivo nas duas cultivares. Diferente do amplamente registrado, identificaramse somaclones em segmentos apicais caulinares e nodais originados de organogenese direta em 'Asterix' e '.Macaca'. Palavras-chave: variacao somaclonal, estabilidade fenotipica, organogenese direta, numero de subcultivos. |
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ISSN: | 0103-8478 |
DOI: | 10.1590/0103-8478cr20140557 |