Simulation of a stream of photon counts in single-molecule fluorescence fluctuation spectroscopy

We present a simulation model for a stream of photon counts that can be detected in fluorescence fluctuation spectroscopy systems from diffusing molecules undergoing spontaneous transitions between radiative and nonradiative states. Using our model, we can obtain various characteristics of a random...

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Veröffentlicht in:	Journal of applied spectroscopy 2012, Vol.78 (6), p.892-898
Hauptverfasser:	Shingaryov, I. P., Skakun, V. V., Apanasovich, V. V.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Analytical Chemistry Atomic/Molecular Structure and Spectra Fluorescence Light Physics Physics and Astronomy Spectrum analysis
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	We present a simulation model for a stream of photon counts that can be detected in fluorescence fluctuation spectroscopy systems from diffusing molecules undergoing spontaneous transitions between radiative and nonradiative states. Using our model, we can obtain various characteristics of a random stream of photon counts which allows us to test fluorescence fluctuation spectroscopy methods and to predict the results of full-scale experiments.
ISSN:	0021-9037 1573-8647
DOI:	10.1007/s10812-012-9549-7