NKX2-[5.sup.eGFP/w] hESCs for isolation of human cardiac progenitors and cardiomyocytes

NKX2-[5.sup.eGFP/w] hESCs for isolation of human cardiac progenitors and cardiomyocytes

NKX2-5 is expressed in the heart throughout life. We targeted eGFP sequences to the NKX2-5 locus of human embryonic stem cells (hESCs); NKX2-[5.sup.eGFP/w] hESCs facilitate quantification of cardiac differentiation, purification of hESC-derived committed cardiac progenitor cells (hESC-CPCs) and card...

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Veröffentlicht in:Nature Methods 2011, Vol.8 (12), p.1037
Hauptverfasser: Elliott, David A, Braam, Stefan R, Koutsis, Katerina, Ng, Elizabeth S, Jenny, Robert, Lagerqvist, Ebba L, Biben, Christine, Hatzistavrou, Tanya, Hirst, Claire E, Yu, Qing C, Skelton, Rhys J.P, Oostwaard, Dorien Ward-van, Lim, Sue Mei, Khammy, Ouda, Li, Xueling, Hawes, Susan M, Davis, Richard P, Goulburn, Adam L, Passier, Robert, Prall, Owen W.J, Haynes, John M, Pouton, Colin W, Kaye, David M, Mummery, Christine L, Elefanty, Andrew G, Stanley, Edouard G
Format: Report
Sprache:eng
Schlagworte:
Embryonic stem cells
Gene expression
Heart cells
Physiological aspects
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Zusammenfassung:NKX2-5 is expressed in the heart throughout life. We targeted eGFP sequences to the NKX2-5 locus of human embryonic stem cells (hESCs); NKX2-[5.sup.eGFP/w] hESCs facilitate quantification of cardiac differentiation, purification of hESC-derived committed cardiac progenitor cells (hESC-CPCs) and cardiomyocytes (hESC-CMs) and the standardization of differentiation protocols. We used NKX2-5 [eGFP.sup.+] cells to identify VCAM1 and SIRPA as cell-surface markers expressed in cardiac lineages.
ISSN:1548-7091
DOI:10.1038/NMETH.1740