Maternal effect in sulfur amino acids content expression in common bean grains/Efeito materno na expressao dos teores de aminoacidos sulfurados em graos de feijao

The genetic of the sulfur amino acids content has not been sufficiently evaluated in common bean. The objective of this research was to investigate the existence of maternal effect in sulfur amino acids content (methionine and cystein) of common bean grains. The controlled crossings were performed a...

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Veröffentlicht in:Ciência rural 2009-09, Vol.39 (6), p.1884
Hauptverfasser: Londero, Patricia Medianeira Grigoletto, Ribeiro, Nerineia Dalfollo, Cerutti, Taiguer, Maziero, Sandra Maria, Rosa, Daniele Piano, Rosa, Simone Saydelles da
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Zusammenfassung:The genetic of the sulfur amino acids content has not been sufficiently evaluated in common bean. The objective of this research was to investigate the existence of maternal effect in sulfur amino acids content (methionine and cystein) of common bean grains. The controlled crossings were performed among the cultivars 'BRS Valente' x 'IAPAR 44' and 'TPS Nobre' x 'Minuano'. The [F.sub.1], [F.sub.1] reciprocal, [F.sub.2] and [F.sub.2] reciprocal generations were obtained for each hybrid combination. The amino acid content was determined by high performance liquid chromatography (HPLC-UV). The methione content varied from 0.79g for 16g N MS ('BRS Valente') to 1.09g for 16g N MS (F2 generation) and the cystein content varied from 0.76g for 16g N MS ([F.sub.2] generation) to 1.43g for 16g N MS ('Minuano'). Genetic variability was observed between the parents, but was there was no maternal effect in the methione and cystein content. The selection for higher sulfur amino acids content can be performed in [F.sub.2] seeds, because the generation of the embryo is [F.sub.2]. Key words: Phaseolus vulgaris L., genetic variability, early generation, selection. A genetica do teor de aminoacidos sulfurados em feijao nao tem sido suficientemente avaliada, por isso o objetivo deste trabalho foi verificar se ocorre efeito materno na expressao dos teores de aminoacidos sulfurados (metionina e cisteina) em graos de feijao. Para isso, foram realizados cruzamentos controlados entre as cultivares 'BRS Valente' x 'IAPAR 44' e 'TPS Nobre' x 'Minuano', e as geracoes [F.sub.1], [F.sub.1] reciproco, [F.sub.2] e [F.sub.2] reciproco foram obtidas para cada combinacao hibrida. Os aminoacidos sulfurados foram determinados por cromatografia liquida de alto desempenho (HPLC-UV). O teor de metionina variou de 0,79g por 16g de N da materia seca--MS ('BRS Valente') a 1,09g por 16g de N da MS (geracao [F.sub.2]), e o teor de cisteina variou de 0,76g por 16g de N da MS (geracao [F.sub.2]) a 1,43g por 16g de N da MS ('Minuano'). Variabilidade genetica foi observada entre os genitores, porem nao ocorre efeito materno na expressao dos teores de metionina e de cisteina em feijao. A selecao para maior teor de aminoacidos sulfurados em graos de feijao deve ser realizada em sementes [F.sub.2], pois a geracao do embriao e [F.sub.2]. Palavras-chave: Phaseolus vulgaris L., variabilidade genetica, geracoes precoces, selecao.
ISSN:0103-8478