Detection and prognostic relevance of circulating tumour cells

We aimed to study the prevalence of CTCs in breast cancer (BC) patients undergoing neoadjuvant or palliative therapy with a label-free microfluidic platform (ClearCell FX), and its prognostic relevance in metastatic BC (mBC). Peripheral blood samples were collected from 108 BC patients before starti...

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Veröffentlicht in:PloS one 2019-09, Vol.14 (9), p.e0221305
Hauptverfasser: Yap, Yoon-Sim, Leong, Man Chun, Chua, Yong Wei, Loh, Kiley Wei Jen, Lee, Guek Eng, Lim, Elaine Hsuen, Dent, Rebecca, Ng, Raymond Chee Hui, Lim, John Heng-Chi, Singh, Garima, Tan, Angela, Guan, Guofeng, Wu, Andrew, Lee, Yi Fang, Bhagat, Ali Asgar S, Lim, Darren Wan-Teck
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Sprache:eng
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Zusammenfassung:We aimed to study the prevalence of CTCs in breast cancer (BC) patients undergoing neoadjuvant or palliative therapy with a label-free microfluidic platform (ClearCell FX), and its prognostic relevance in metastatic BC (mBC). Peripheral blood samples were collected from 108 BC patients before starting a new line of treatment ("baseline"), majority of whom had mBC (76/108; 70.4%). CTCs were retrieved by dean flow fractionation that enriched for larger cells, and enumerated using immunofluorescence-based staining. Progression-free survival (PFS) in mBC patients was analysed using Kaplan-Meier method; cox proportional hazard models were used for univariable and multivariable analyses. The detection rate of CTCs before starting a new line of treatment was 75.9% (n = 108; median: 8 CTCs/7.5 ml blood) at a cut off of [greater than or equal to]2 CTCs. PFS was inferior for mBC patients with baseline CTC count [greater than or equal to]5 CTCs/7.5 ml blood vs. those with < 5 CTCs/7.5 ml blood (median PFS: 4.3 vs. 7.0 months; p-value: 0.037). The prognostic relevance of CTCs was most significant in patients with HER2.sup.- mBC (median PFS: 4.1 vs. 8.3 months; p-value: 0.032), luminal (HR+HER2-) subtype (median PFS: 4.2 vs. 8.3 months; p-value: 0.048), and patients who had one or more prior treatments (median PFS: 4.2 vs. 7.0 months; p-value: 0.02). On multivariable analysis, baseline CTC level (hazard ratio (HR): 1.84, p-value: 0.02) and pre-treatment status (HR: 1.87, p-value: 0.05) were independent predictors of PFS. This work demonstrates the prognostic significance of CTCs in mBC detected using a label-free size-based enrichment platform.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0221305