Analysis of anti-apoptotic protein

In assisted reproductive technology (ART), researchers have tried to predict in vitro fertilization (IVF) outcomes depending on follicular cells apoptosis. Previous reports suggested the B-cell lymphoma-extra-large (BCL-XL) as an apoptosis inhibitor in mammalian ovaries. Therefore, the present research aimed to correlate BCL-XL expression in blood and concentration in follicular fluid (FF) with some outcomes of IVF. A prospective case-control study on infertile women (n=80, mean age= 31.18[+ or -] 6.04 years) who underwent an IVF program at Kamal Al-Samarai Hospital (Baghdad/Iraq), July 2021- January 2022. All women were split based on pregnancy outcome into two groups: the non-pregnant group (n=40) and the pregnant group (n=40). Samples of FF and blood were assembled at oocyte retrieval time. The BCL-XL mRNA expression was assessed with the Real-Time Quantitative Reverse Transcription-polymerase chain reaction (Real-Time qRT-PCR) technique, whereas the BCL-XL concentration in FF was investigated by sandwich enzyme-linked immunosorbent assay (ELISA). The BCL-XL concentration in FF from pregnant group (3.90 ng/ml [+ or -]1.16) raised significantly (P = 0.00) compared with non-pregnant group (1.90 ng/ml [+ or -] 0.63). Similarly, the BCL-XL mRNA expression in blood from pregnant group (0.73 [+ or -]0.31) raised significantly (P = 0.00) compared with non-pregnant group (0.34[+ or -] 0.12). In addition, FF BCL-XL concentration associated significantly with fertilization rate (r= 0.399, P=0.048). The research proposed that the elevation of anti-apoptotic BCL-XL might assist in diminished apoptosis in pregnant women who underwent IVF treatment. (Afr J Reprod Health 2022; 26[10]: 63-71). Keywords: In vitro fertilization, apoptosis, BCL-XL, follicular fluid, pregnancy Dans le domaine de la technologie de procreation assistee (ART), les chercheurs ont tente de predire les resultats de la fecondation in vitro (FIV) en fonction de l'apoptose des cellules folliculaires. Des rapports anterieurs ont suggere le lymphome a cellules B extra-large (BCL-XL) comme inhibiteur de l'apoptose dans les ovaires de mammiferes. Par consequent, la presente recherche visait a correler l'expression de BCL-XL dans le sang et la concentration dans le liquide folliculaire (FF) avec certains resultats de la FIV. Une etude prospective cas-temoins sur des femmes infertiles (n = 80, age moyen = 31,18 [+ or -] 6,04 ans) ayant suivi un programme de FIV a l'hopital Kamal Al-Samarai (Bagdad/Irak),