A method for rapid analysis of the root hydrotropic response in

The system for analyzing the hydrotropic curvature with a moisture gradient in wild-type roots was modified. Optimal conditions were determined for detecting a hydrotropic curvature of 90° just after 4 h of stimulation. This system only requires 15 ml of a solution of K CO with a density of 1.48 g·m...

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Veröffentlicht in:BioTechniques 2019-03, Vol.66 (3), p.154-158
Hauptverfasser: Campos, María E, Cassab, Gladys I
Format: Artikel
Sprache:eng
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Zusammenfassung:The system for analyzing the hydrotropic curvature with a moisture gradient in wild-type roots was modified. Optimal conditions were determined for detecting a hydrotropic curvature of 90° just after 4 h of stimulation. This system only requires 15 ml of a solution of K CO with a density of 1.48 g·ml to generate a rapid moisture gradient inside a square Petri dish without decreasing root growth. In this, the root growth rate observed in hydrostimulated wild-type and mutant, utilized as a negative control, increases sixfold compared with those roots examined using the former method.
ISSN:0736-6205
1940-9818
DOI:10.2144/btn-2018-0173