Genotyping of

Genotyping of

Fluorescence resonance energy transfer (FRET) genotyping has been well established for the rapid assessment of single nucleotide polymorphisms (SNPs) and deletions. A design is presented that allows the typing of short tandem oligo repeat sequences using the LightTyper/LightCycler system. The protoc...

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Veröffentlicht in:BioTechniques 2009-01, Vol.46 (1), p.58-60
Hauptverfasser: Intemann, Christopher D, Thye, Thorsten, Sievertsen, Jürgen, Owusu-Dabo, Ellis, Horstmann, Rolf D, Meyer, Christian G
Format: Artikel
Sprache:eng
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Zusammenfassung:Fluorescence resonance energy transfer (FRET) genotyping has been well established for the rapid assessment of single nucleotide polymorphisms (SNPs) and deletions. A design is presented that allows the typing of short tandem oligo repeat sequences using the LightTyper/LightCycler system. The protocol was evaluated and applied to the typing of a tetranucleotide promoter repeat of the human gene encoding the immunity-related GTPase family, M (IRGM) molecule in >2000 individuals from Ghana, West Africa.
ISSN:0736-6205
1940-9818
DOI:10.2144/000113045