Characterization of JKT-7000, an orbivirus which grows in Aedes albopictus mosquito cells: evidence pointing to a minor virion protein, VP6, as the RNA guanylyltransferase

Characterization of JKT-7000, an orbivirus which grows in Aedes albopictus mosquito cells: evidence pointing to a minor virion protein, VP6, as the RNA guanylyltransferase

JKT-7400 virus, an orbivirus originally isolated from Culex mosquitos, was plaque purified and adapted to Aedes albopictus mosquito cells. Conditions which enhance viral cytopathic effect and optimize plaque formation are described. In contrast to bluetongue virus, the prototype orbivirus, no replic...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Virology (New York, N.Y.) N.Y.), 1997-02, Vol.228 (1)
Hauptverfasser: Liao, H.J. (UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ.), Stollar, V
Format: Artikel
Sprache:eng
Schlagworte:
AEDES ALBOPICTUS
ORBIVIRUS
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue 1
container_start_page
container_title Virology (New York, N.Y.)
container_volume 228
creator Liao, H.J. (UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ.)
Stollar, V
description JKT-7400 virus, an orbivirus originally isolated from Culex mosquitos, was plaque purified and adapted to Aedes albopictus mosquito cells. Conditions which enhance viral cytopathic effect and optimize plaque formation are described. In contrast to bluetongue virus, the prototype orbivirus, no replication of JKT-7400 virus in vertebrate cells was observed. The core particle of JKT-7400 virus contains 10 segments of dsRNA and three minor proteins, VP1, VP4, and VP6. The inner shell contains two major proteins, VP2 and VP7, and the outer shell consists of the other two major proteins, VP3 and VP5. Evidence is presented suggesting that the viral protein associated with the capping of virus mRNA, i.e., the guanylyltransferase, is VP6, one of the core proteins
format Article
fullrecord <record><control><sourceid>fao</sourceid><recordid>TN_cdi_fao_agris_US9717776</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>US9717776</sourcerecordid><originalsourceid>FETCH-fao_agris_US97177763</originalsourceid><addsrcrecordid>eNp9jd1Kw0AQhRdRMP68gFfzAA1s0pK13pWiiIKItr0t03SSjGx3486mpb6SL-kKXnt1-PgO55yorNDTKtfjSXGqMq0nZV7dluW5uhD50ImN0Zn6nncYsI4U-Asjewe-gafnRW5SZQSYOGx4z2EQOHRcd9AGfxBgBzPakgDaje-5jsnvvHwOHD3UZK3cAe15S64m6D27yK6F5BB27HyANPn71gcfid0IVq9VuhOIHcHbywzaAd3RHm0M6KShgEJX6qxBK3T9l5fq5uF-MX_MG_RrbAPLevk-NYUxphr_K38AuElZdg</addsrcrecordid><sourcetype>Publisher</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Characterization of JKT-7000, an orbivirus which grows in Aedes albopictus mosquito cells: evidence pointing to a minor virion protein, VP6, as the RNA guanylyltransferase</title><source>Elsevier ScienceDirect Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Liao, H.J. (UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ.) ; Stollar, V</creator><creatorcontrib>Liao, H.J. (UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ.) ; Stollar, V</creatorcontrib><description>JKT-7400 virus, an orbivirus originally isolated from Culex mosquitos, was plaque purified and adapted to Aedes albopictus mosquito cells. Conditions which enhance viral cytopathic effect and optimize plaque formation are described. In contrast to bluetongue virus, the prototype orbivirus, no replication of JKT-7400 virus in vertebrate cells was observed. The core particle of JKT-7400 virus contains 10 segments of dsRNA and three minor proteins, VP1, VP4, and VP6. The inner shell contains two major proteins, VP2 and VP7, and the outer shell consists of the other two major proteins, VP3 and VP5. Evidence is presented suggesting that the viral protein associated with the capping of virus mRNA, i.e., the guanylyltransferase, is VP6, one of the core proteins</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><language>eng</language><subject>AEDES ALBOPICTUS ; ORBIVIRUS</subject><ispartof>Virology (New York, N.Y.), 1997-02, Vol.228 (1)</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781</link.rule.ids></links><search><creatorcontrib>Liao, H.J. (UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ.)</creatorcontrib><creatorcontrib>Stollar, V</creatorcontrib><title>Characterization of JKT-7000, an orbivirus which grows in Aedes albopictus mosquito cells: evidence pointing to a minor virion protein, VP6, as the RNA guanylyltransferase</title><title>Virology (New York, N.Y.)</title><description>JKT-7400 virus, an orbivirus originally isolated from Culex mosquitos, was plaque purified and adapted to Aedes albopictus mosquito cells. Conditions which enhance viral cytopathic effect and optimize plaque formation are described. In contrast to bluetongue virus, the prototype orbivirus, no replication of JKT-7400 virus in vertebrate cells was observed. The core particle of JKT-7400 virus contains 10 segments of dsRNA and three minor proteins, VP1, VP4, and VP6. The inner shell contains two major proteins, VP2 and VP7, and the outer shell consists of the other two major proteins, VP3 and VP5. Evidence is presented suggesting that the viral protein associated with the capping of virus mRNA, i.e., the guanylyltransferase, is VP6, one of the core proteins</description><subject>AEDES ALBOPICTUS</subject><subject>ORBIVIRUS</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNp9jd1Kw0AQhRdRMP68gFfzAA1s0pK13pWiiIKItr0t03SSjGx3486mpb6SL-kKXnt1-PgO55yorNDTKtfjSXGqMq0nZV7dluW5uhD50ImN0Zn6nncYsI4U-Asjewe-gafnRW5SZQSYOGx4z2EQOHRcd9AGfxBgBzPakgDaje-5jsnvvHwOHD3UZK3cAe15S64m6D27yK6F5BB27HyANPn71gcfid0IVq9VuhOIHcHbywzaAd3RHm0M6KShgEJX6qxBK3T9l5fq5uF-MX_MG_RrbAPLevk-NYUxphr_K38AuElZdg</recordid><startdate>19970203</startdate><enddate>19970203</enddate><creator>Liao, H.J. (UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ.)</creator><creator>Stollar, V</creator><scope>FBQ</scope></search><sort><creationdate>19970203</creationdate><title>Characterization of JKT-7000, an orbivirus which grows in Aedes albopictus mosquito cells: evidence pointing to a minor virion protein, VP6, as the RNA guanylyltransferase</title><author>Liao, H.J. (UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ.) ; Stollar, V</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-fao_agris_US97177763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>AEDES ALBOPICTUS</topic><topic>ORBIVIRUS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liao, H.J. (UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ.)</creatorcontrib><creatorcontrib>Stollar, V</creatorcontrib><collection>AGRIS</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liao, H.J. (UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ.)</au><au>Stollar, V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of JKT-7000, an orbivirus which grows in Aedes albopictus mosquito cells: evidence pointing to a minor virion protein, VP6, as the RNA guanylyltransferase</atitle><jtitle>Virology (New York, N.Y.)</jtitle><date>1997-02-03</date><risdate>1997</risdate><volume>228</volume><issue>1</issue><issn>0042-6822</issn><eissn>1096-0341</eissn><abstract>JKT-7400 virus, an orbivirus originally isolated from Culex mosquitos, was plaque purified and adapted to Aedes albopictus mosquito cells. Conditions which enhance viral cytopathic effect and optimize plaque formation are described. In contrast to bluetongue virus, the prototype orbivirus, no replication of JKT-7400 virus in vertebrate cells was observed. The core particle of JKT-7400 virus contains 10 segments of dsRNA and three minor proteins, VP1, VP4, and VP6. The inner shell contains two major proteins, VP2 and VP7, and the outer shell consists of the other two major proteins, VP3 and VP5. Evidence is presented suggesting that the viral protein associated with the capping of virus mRNA, i.e., the guanylyltransferase, is VP6, one of the core proteins</abstract></addata></record>
fulltext fulltext
identifier ISSN: 0042-6822
ispartof Virology (New York, N.Y.), 1997-02, Vol.228 (1)
issn 0042-6822
1096-0341
language eng
recordid cdi_fao_agris_US9717776
source Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals
subjects AEDES ALBOPICTUS
ORBIVIRUS
title Characterization of JKT-7000, an orbivirus which grows in Aedes albopictus mosquito cells: evidence pointing to a minor virion protein, VP6, as the RNA guanylyltransferase
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T17%3A39%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-fao&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Characterization%20of%20JKT-7000,%20an%20orbivirus%20which%20grows%20in%20Aedes%20albopictus%20mosquito%20cells:%20evidence%20pointing%20to%20a%20minor%20virion%20protein,%20VP6,%20as%20the%20RNA%20guanylyltransferase&rft.jtitle=Virology%20(New%20York,%20N.Y.)&rft.au=Liao,%20H.J.%20(UMDNJ-Robert%20Wood%20Johnson%20Medical%20School,%20Piscataway,%20NJ.)&rft.date=1997-02-03&rft.volume=228&rft.issue=1&rft.issn=0042-6822&rft.eissn=1096-0341&rft_id=info:doi/&rft_dat=%3Cfao%3EUS9717776%3C/fao%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true