Characterization of JKT-7000, an orbivirus which grows in Aedes albopictus mosquito cells: evidence pointing to a minor virion protein, VP6, as the RNA guanylyltransferase

JKT-7400 virus, an orbivirus originally isolated from Culex mosquitos, was plaque purified and adapted to Aedes albopictus mosquito cells. Conditions which enhance viral cytopathic effect and optimize plaque formation are described. In contrast to bluetongue virus, the prototype orbivirus, no replication of JKT-7400 virus in vertebrate cells was observed. The core particle of JKT-7400 virus contains 10 segments of dsRNA and three minor proteins, VP1, VP4, and VP6. The inner shell contains two major proteins, VP2 and VP7, and the outer shell consists of the other two major proteins, VP3 and VP5. Evidence is presented suggesting that the viral protein associated with the capping of virus mRNA, i.e., the guanylyltransferase, is VP6, one of the core proteins