Adrenocorticotropic hormone dose response and some physiological effects of transporation on pregnant Brahman cattle

The appropriate dose and the ability of exogenous ACTH to mimic the physiological effects of a real stressor need to be determined. In Exp. 1, 25 pregnant Brahman heifers were injected i.v. with either 0 (saline), 0.125, 0.25, 0.5, or 1 IU of ACTH/kg BW. Plasma cortisol was determined in blood samples collected during a 5-h period, and an integrated cortisol response was calculated for each cow. The greater the dose of ACTH, the greater was the integrated cortisol response (P 0.001). However, peak plasma cortisol in response to the four doses of ACTH did not differ (P 0.6). The plasma cortisol concentrations returned to baseline more slowly in those cows receiving the greater doses of ACTH, making their integrated areas of response greater. In Exp. 2, pregnant Brahman cows were either transported 48 km (n