Interaction between caulifower mosaic virus inclusion body protein and capsid protein: implications for viral assembly

Interaction between caulifower mosaic virus inclusion body protein and capsid protein: implications for viral assembly

The cauliflower mosaic virus (CaMV) inclusion body protein (pVI) is able to specifically interact with the viral capsid precursor protein (pIV). By using the yeast two-hybrid system and a blot assay, the pIV region required for the recognition of pVI was mapped to the lysine-rich domain. This region...

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Veröffentlicht in:Virology (New York, N.Y.) N.Y.), 1996-03, Vol.217 (1)
Hauptverfasser: Himmelbach, A. (Technische Universitat Munchen, Munchen, Germany.), Chapdelaine, Y, Hohn, T
Format: Artikel
Sprache:eng
Schlagworte:
ADN
CAULIMOVIRUS MOSAICO DEL COLIFLOR
CAULIMOVIRUS MOSAIQUE DU CHOU FLEUR
CITOPLASMA
CYTOPLASME
MUTACION
MUTATION
PROTEINAS
PROTEINAS AGLUTINANTES
PROTEINE
PROTEINE DE LIAISON
VIRUS DE LAS PLANTAS
VIRUS DES VEGETAUX
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Zusammenfassung:The cauliflower mosaic virus (CaMV) inclusion body protein (pVI) is able to specifically interact with the viral capsid precursor protein (pIV). By using the yeast two-hybrid system and a blot assay, the pIV region required for the recognition of pVI was mapped to the lysine-rich domain. This region of only 48 amino acids when fused to dihydrofolate reductase (DHFR) mediated pVI and DNA binding in vitro. Competition experiments confirmed that pVI and DNA bind to the same region of pIV. Since pVI is absent from the mature virus, models are discussed in which pVI plays an accessory role in CaMV assembly, in addition to its function in transactivating translation
ISSN:0042-6822
1096-0341