hybrid technique for staining meiotic nuclei of basidiomycetes

hybrid technique for staining meiotic nuclei of basidiomycetes

A hybrid nuclear staining procedure used on the basidiomycete Schizophyllum commune produced squash preparations of sufficient density for effective brightfield photomicrography. Procedure: Tissue is fixed in Newcomer's solution, rehydrated in a graded ethanol series, rinsed, hydrolyzed in 1 N...

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Veröffentlicht in:Biotechnic & histochemistry 1976-01, Vol.51 (2), p.103-105
1. Verfasser: Sundberg, W.J
Format: Artikel
Sprache:eng
Schlagworte:
Agaricales - ultrastructure
Cell Nucleus - ultrastructure
Chromosomes - ultrastructure
fungi
Hematoxylin
Meiosis
Schizophyllum - ultrastructure
Schizophyllum commune
Staining and Labeling
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Zusammenfassung:A hybrid nuclear staining procedure used on the basidiomycete Schizophyllum commune produced squash preparations of sufficient density for effective brightfield photomicrography. Procedure: Tissue is fixed in Newcomer's solution, rehydrated in a graded ethanol series, rinsed, hydrolyzed in 1 N HC; at 60 C, washed, mordanted in 4% iron alum, rinsed again, and stained with aceto-iron-hematoxylin prior to mounting and squashing.
ISSN:0038-9153
1052-0295
1473-7760
DOI:10.3109/10520297609116679