Analysis of legume oligosaccharides by high-resolution gas chromatography
The suitability of high-resolution gas chromatography (HGRC) for the analysis of the raffinose family oligosaccharides (raffinose, stachyose, verbascose) was investigated. Aqueous methanol (80%) extracts of pea flour were dried and derivatized with either trimethyl- imidazole or N-methyl-bis(trifluo...
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| Veröffentlicht in: | Journal of Chromatography A 1992-10, Vol.623 (1), p.186-190 |
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| container_title | Journal of Chromatography A |
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| creator | Karoutis, Apostolos I. Tyler, Robert T. Slater, George P. |
| description | The suitability of high-resolution gas chromatography (HGRC) for the analysis of the raffinose family oligosaccharides (raffinose, stachyose, verbascose) was investigated. Aqueous methanol (80%) extracts of pea flour were dried and derivatized with either trimethyl- imidazole or N-methyl-bis(trifluoroacetamide). Separation of the sugar derivatives was achieved uitilizing a 10-m DB5-60W capillary column. The effects of carrier gas (He) flow-rate and split ratio on resolution and reproducibility were studied. HRGC analysis was characterized by excellent resolution and satisfactory reproducibility, and proved to be a rapid, sensitive method for quantitation of oligosaccharides in pea flours. |
| doi_str_mv | 10.1016/0021-9673(92)85317-M |
| format | Article |
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Aqueous methanol (80%) extracts of pea flour were dried and derivatized with either trimethyl- imidazole or N-methyl-bis(trifluoroacetamide). Separation of the sugar derivatives was achieved uitilizing a 10-m DB5-60W capillary column. The effects of carrier gas (He) flow-rate and split ratio on resolution and reproducibility were studied. HRGC analysis was characterized by excellent resolution and satisfactory reproducibility, and proved to be a rapid, sensitive method for quantitation of oligosaccharides in pea flours.</description><identifier>ISSN: 0021-9673</identifier><identifier>DOI: 10.1016/0021-9673(92)85317-M</identifier><identifier>PMID: 1452628</identifier><identifier>CODEN: JOCRAM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>analysis ; Biological and medical sciences ; Chromatography, Gas - standards ; Fabaceae - chemistry ; Flour - analysis ; Food industries ; Fruit and vegetable industries ; Fundamental and applied biological sciences. 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Aqueous methanol (80%) extracts of pea flour were dried and derivatized with either trimethyl- imidazole or N-methyl-bis(trifluoroacetamide). Separation of the sugar derivatives was achieved uitilizing a 10-m DB5-60W capillary column. The effects of carrier gas (He) flow-rate and split ratio on resolution and reproducibility were studied. HRGC analysis was characterized by excellent resolution and satisfactory reproducibility, and proved to be a rapid, sensitive method for quantitation of oligosaccharides in pea flours.</description><subject>analysis</subject><subject>Biological and medical sciences</subject><subject>Chromatography, Gas - standards</subject><subject>Fabaceae - chemistry</subject><subject>Flour - analysis</subject><subject>Food industries</subject><subject>Fruit and vegetable industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gas chromatography</subject><subject>Indicators and Reagents</subject><subject>legumes</subject><subject>oligosaccharides</subject><subject>Oligosaccharides - analysis</subject><subject>Oligosaccharides - standards</subject><subject>pea flour</subject><subject>peas</subject><subject>Plants, Medicinal</subject><subject>Trifluoroacetic Acid</subject><subject>Trimethylsilyl Compounds</subject><issn>0021-9673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kD1PwzAQhj2ASin8AxAZGGAI-COxkwWpqvio1IoBOluOc06M0riyU6T-e1JSlY3ppLvnPd09CF0R_EAw4Y8YUxLnXLC7nN5nKSMiXp6g8bF9hs5D-MKYCCzoCI1IklJOszGaT1vV7IINkTNRA9V2DZFrbOWC0rpW3pYQomIX1baqYw_BNdvOujaqVIh07d1ada7yalPvLtCpUU2Ay0OdoNXL8-fsLV68v85n00Wsmci72BCqCs4541ylIuNUlxwI1xmQUpiCEcCiKBlkiUlxzk2ai4QonuU4xSVAyiYoGfZq70LwYOTG27XyO0mw3MuQ-6_l_muZU_krQy772PUQ22yLNZR_ocFEP789zFXQqjFetdqGI5awnPOM9NjNgBnlpKp8j6w-KCasV8uxEKwnngYCegnfFrwM2kKrobQedCdLZ_-_9AcYO4ap</recordid><startdate>19921009</startdate><enddate>19921009</enddate><creator>Karoutis, Apostolos I.</creator><creator>Tyler, Robert T.</creator><creator>Slater, George P.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19921009</creationdate><title>Analysis of legume oligosaccharides by high-resolution gas chromatography</title><author>Karoutis, Apostolos I. ; Tyler, Robert T. ; Slater, George P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c379t-f12ab666366a57862cd6e16c8e1d7fb31e07bd3e84f5096f59741a689050dee53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>analysis</topic><topic>Biological and medical sciences</topic><topic>Chromatography, Gas - standards</topic><topic>Fabaceae - chemistry</topic><topic>Flour - analysis</topic><topic>Food industries</topic><topic>Fruit and vegetable industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gas chromatography</topic><topic>Indicators and Reagents</topic><topic>legumes</topic><topic>oligosaccharides</topic><topic>Oligosaccharides - analysis</topic><topic>Oligosaccharides - standards</topic><topic>pea flour</topic><topic>peas</topic><topic>Plants, Medicinal</topic><topic>Trifluoroacetic Acid</topic><topic>Trimethylsilyl Compounds</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Karoutis, Apostolos I.</creatorcontrib><creatorcontrib>Tyler, Robert T.</creatorcontrib><creatorcontrib>Slater, George P.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Karoutis, Apostolos I.</au><au>Tyler, Robert T.</au><au>Slater, George P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of legume oligosaccharides by high-resolution gas chromatography</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr</addtitle><date>1992-10-09</date><risdate>1992</risdate><volume>623</volume><issue>1</issue><spage>186</spage><epage>190</epage><pages>186-190</pages><issn>0021-9673</issn><coden>JOCRAM</coden><abstract>The suitability of high-resolution gas chromatography (HGRC) for the analysis of the raffinose family oligosaccharides (raffinose, stachyose, verbascose) was investigated. Aqueous methanol (80%) extracts of pea flour were dried and derivatized with either trimethyl- imidazole or N-methyl-bis(trifluoroacetamide). Separation of the sugar derivatives was achieved uitilizing a 10-m DB5-60W capillary column. The effects of carrier gas (He) flow-rate and split ratio on resolution and reproducibility were studied. HRGC analysis was characterized by excellent resolution and satisfactory reproducibility, and proved to be a rapid, sensitive method for quantitation of oligosaccharides in pea flours.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>1452628</pmid><doi>10.1016/0021-9673(92)85317-M</doi><tpages>5</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | analysis Biological and medical sciences Chromatography, Gas - standards Fabaceae - chemistry Flour - analysis Food industries Fruit and vegetable industries Fundamental and applied biological sciences. Psychology gas chromatography Indicators and Reagents legumes oligosaccharides Oligosaccharides - analysis Oligosaccharides - standards pea flour peas Plants, Medicinal Trifluoroacetic Acid Trimethylsilyl Compounds |
| title | Analysis of legume oligosaccharides by high-resolution gas chromatography |
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