Analysis of legume oligosaccharides by high-resolution gas chromatography

The suitability of high-resolution gas chromatography (HGRC) for the analysis of the raffinose family oligosaccharides (raffinose, stachyose, verbascose) was investigated. Aqueous methanol (80%) extracts of pea flour were dried and derivatized with either trimethyl- imidazole or N-methyl-bis(trifluo...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of Chromatography A 1992-10, Vol.623 (1), p.186-190
Hauptverfasser:	Karoutis, Apostolos I., Tyler, Robert T., Slater, George P.
Format:	Artikel
Sprache:	eng
Schlagworte:	analysis Biological and medical sciences Chromatography, Gas - standards Fabaceae - chemistry Flour - analysis Food industries Fruit and vegetable industries Fundamental and applied biological sciences. Psychology gas chromatography Indicators and Reagents legumes oligosaccharides Oligosaccharides - analysis Oligosaccharides - standards pea flour peas Plants, Medicinal Trifluoroacetic Acid Trimethylsilyl Compounds
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The suitability of high-resolution gas chromatography (HGRC) for the analysis of the raffinose family oligosaccharides (raffinose, stachyose, verbascose) was investigated. Aqueous methanol (80%) extracts of pea flour were dried and derivatized with either trimethyl- imidazole or N-methyl-bis(trifluoroacetamide). Separation of the sugar derivatives was achieved uitilizing a 10-m DB5-60W capillary column. The effects of carrier gas (He) flow-rate and split ratio on resolution and reproducibility were studied. HRGC analysis was characterized by excellent resolution and satisfactory reproducibility, and proved to be a rapid, sensitive method for quantitation of oligosaccharides in pea flours.
ISSN:	0021-9673
DOI:	10.1016/0021-9673(92)85317-M