Comparison of Routine Bench and Molecular Diagnostic Methods in Identification of Burkholderia pseudomallei

This study compared the identification of Burkholderia pseudomallei with that of related organisms. Bench tests and latex agglutination were compared with molecular identification. Using bench tests and latex agglutination alone, 100% (30/30) of B. pseudomallei isolates were correctly identified. Am...

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Veröffentlicht in:	Journal of Clinical Microbiology 2009-05, Vol.47 (5), p.1578-1580
Hauptverfasser:	Hodgson, Kelly, Engler, Cathy, Govan, Brenda, Ketheesan, Natkunam, Norton, Robert
Format:	Artikel
Sprache:	eng
Schlagworte:	Amoxicillin-Potassium Clavulanate Combination - pharmacology Anti-Bacterial Agents - pharmacology Bacteriological Techniques - methods Bacteriology Biological and medical sciences Burkholderia pseudomallei Burkholderia pseudomallei - isolation & purification Fundamental and applied biological sciences. Psychology Humans Latex Fixation Tests - methods Melioidosis - diagnosis Microbial Sensitivity Tests Microbiology Miscellaneous Molecular Diagnostic Techniques - methods Oxidoreductases - analysis Reverse Transcriptase Polymerase Chain Reaction - methods Sensitivity and Specificity
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Beschreibung
Zusammenfassung:	This study compared the identification of Burkholderia pseudomallei with that of related organisms. Bench tests and latex agglutination were compared with molecular identification. Using bench tests and latex agglutination alone, 100% (30/30) of B. pseudomallei isolates were correctly identified. Amoxicillin-clavulanate susceptibility testing was also a good and simple discriminatory test.
ISSN:	0095-1137 1098-660X
DOI:	10.1128/JCM.02507-08