Differentiation of F1 hybrids P. nigra J. F. Arnold x P. sylvestris L., P. nigra J. F. Arnold x P. densiflora Siebold et Zucc., P. nigra J. F. Arnold x P. thunbergiana Franco and their parental species by needle volatile composition

The volatile composition of needles from three F1 hard pine hybrids produced by the controlled hybridization and their parental species were researched with gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS) in order to explore the utility of terpenes in hybrid identification (their differentiation from the parental species) as well as confirmation of hybridity. The analysed hybrids were: 1. Pinus nigra J. F. Arnold X Pinus sylvestris L. (= nisy), 2. P. nigra X Pinus densiflora Siebold et Zucc. (= nide) and 3. P. nigra X Pinus thunbergiana Franco (= nith). A total of 55 compounds were identified. All identified compounds were terpenes, except trans-2-hexenal. Three analysed F1 hybrids showed the same qualitative pattern of the needle volatile composition as their parental species. However, there were quantitative differences in several major terpenes. The volatile composition of the needles from the hybrids nisy were equally similar to both parents, the hybrids nide were more similar to the female parent (P. nigra), whereas the hybrids nith were more similar to the male parent (P. thunbergiana). According to the content of germacrene D, as the specific component of P. nigra (female parent of the three analysed F1 hybrids), all hybrids were intermediary in relation to the parental species. The content of delta-3-carene (the specific component of P. sylvestris) in the hybrids nisy was also intermediary. The hybrids nide had a higher content of thunbergol (specific component of P. densiflora) than the other analysed hybrids. In view of the content of beta-pinene, the specific component of P. thunbergiana, the hybrids nith were intermediary to the parental species and that content was considerably higher than in the other analysed hybrids. The intermediary quality of F1 hybrids for these specific components in relation to the parental species confirms their hybrid character. The needle volatile composition analysis as well as the previous morphometric analysis confirm the hybrid character of three F1 hybrids, whose female parent is P. nigra, and male parents are P. sylvestris, P. densiflora, i.e. P. thunbergiana.