Misidentification of Mycobacterium leprae as Mycobacterium intracellulare by the COBAS AMPLICOR M. intracellulare Test

Commercially available nucleic acid probe- and amplification-based systems for detection and differentiation of mycobacteria are widely used in clinical microbiology laboratories. Here we report two cases of human leprosy in which the COBAS AMPLICOR Mycobacterium intracellulare test led to false- po...

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Veröffentlicht in:	Journal of Clinical Microbiology 2005-04, Vol.43 (4), p.1928-1929
Hauptverfasser:	Lefmann, M, Moter, A, Schweickert, B, Göbel, U. B
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Bacterial Typing Techniques Biological and medical sciences Diagnostic Errors DNA, Bacterial - analysis False Positive Reactions Fundamental and applied biological sciences. Psychology Humans Infectious diseases Leprosy - diagnosis Leprosy - microbiology Male Medical sciences Microbiology Middle Aged Mycobacteriology and Aerobic Actinomycetes Mycobacterium avium Complex - classification Mycobacterium avium Complex - genetics Mycobacterium avium Complex - isolation & purification Mycobacterium avium-intracellulare Infection - diagnosis Mycobacterium avium-intracellulare Infection - microbiology Mycobacterium intracellulare Mycobacterium leprae Mycobacterium leprae - classification Mycobacterium leprae - genetics Mycobacterium leprae - isolation & purification Nucleic Acid Amplification Techniques Polymerase Chain Reaction Reagent Kits, Diagnostic RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA
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Beschreibung
Zusammenfassung:	Commercially available nucleic acid probe- and amplification-based systems for detection and differentiation of mycobacteria are widely used in clinical microbiology laboratories. Here we report two cases of human leprosy in which the COBAS AMPLICOR Mycobacterium intracellulare test led to false- positive results. Correct identification of Mycobacterium leprae was possible only by amplification and comparative sequence analysis of the 16S rRNA gene.
ISSN:	0095-1137 1098-660X
DOI:	10.1128/JCM.43.4.1928-1929.2005