Visualization of an endophytic Streptomyces species in wheat seed

Endophytic filamentous actinobacteria were isolated from surface-sterilized roots of wheat plants. Endophytic colonization of germinating wheat seed was examined using one of these endophytes, Streptomyces sp. strain EN27, tagged with the egfp gene. Endophytic colonization was observed from a very e...

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Veröffentlicht in:	Applied and Environmental Microbiology 2003-07, Vol.69 (7), p.4260-4262
Hauptverfasser:	Coombs, J.T, Franco, C.M.M
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria bacterial colonization Biological and medical sciences biological control agents embryo (plant) endophytes endosperm fluorescence Fundamental and applied biological sciences. Psychology fungal antagonists Germination - physiology green fluorescent protein Green Fluorescent Proteins Luminescent Proteins - genetics Luminescent Proteins - metabolism Microbial Ecology Microbiology Microscopy, Confocal root radicle seed-borne bacteria Seeds Seeds - growth & development Seeds - microbiology Streptomyces Streptomyces - genetics Streptomyces - growth & development Streptomyces - metabolism Streptomyces - ultrastructure transgenes Triticum - growth & development Triticum - microbiology Triticum aestivum Visualization Wheat
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Beschreibung
Zusammenfassung:	Endophytic filamentous actinobacteria were isolated from surface-sterilized roots of wheat plants. Endophytic colonization of germinating wheat seed was examined using one of these endophytes, Streptomyces sp. strain EN27, tagged with the egfp gene. Endophytic colonization was observed from a very early stage of plant development with colonization of the embryo, endosperm, and emerging radicle.
ISSN:	0099-2240 1098-5336
DOI:	10.1128/AEM.69.7.4260-4262.2003