Comparison of Primer Sets for Use in Automated Ribosomal Intergenic Spacer Analysis of Aquatic Bacterial Communities: an Ecological Perspective

Two primer sets for automated ribosomal intergenic spacer analysis (ARISA) were used to assess the bacterial community composition (BCC) in Lake Mendota, Wisconsin, over 3 years. Correspondence analysis revealed differences in community profiles generated by different primer sets, but overall ecolog...

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Veröffentlicht in:	Applied and Environmental Microbiology 2007-01, Vol.73 (2), p.659-662
Hauptverfasser:	Jones, Stuart E, Shade, Ashley L, McMahon, Katherine D, Kent, Angela D
Format:	Artikel
Sprache:	eng
Schlagworte:	Automation Bacteria Bacteria - classification Bacteria - genetics Bacteria - growth & development Bacteria - isolation & purification Biological and medical sciences Comparative analysis DNA Primers DNA, Bacterial - analysis DNA, Ribosomal Spacer - analysis Ecosystem Ecosystems Fresh Water - microbiology Freshwater Fundamental and applied biological sciences. Psychology Lakes Microbial Ecology Microbiology Multivariate Analysis RNA, Ribosomal, 16S - genetics RNA, Ribosomal, 23S - genetics Seasons Wisconsin
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Zusammenfassung:	Two primer sets for automated ribosomal intergenic spacer analysis (ARISA) were used to assess the bacterial community composition (BCC) in Lake Mendota, Wisconsin, over 3 years. Correspondence analysis revealed differences in community profiles generated by different primer sets, but overall ecological patterns were conserved in each case. ARISA is a powerful tool for evaluating BCC change through space and time, regardless of the specific primer set used.
ISSN:	0099-2240 1098-5336
DOI:	10.1128/aem.02130-06