Transcription analysis of the prolate-headed lactococcal bacteriophage c2

A detailed transcription map of the prolate-headed lactococcal phage c2 has been constructed. Transcription of about one-third of the genome, encoding 22 open reading frames, began within the first 2 min of infection and produced at least 12 overlapping transcripts that persisted until lysis occurre...

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Veröffentlicht in:Journal of Bacteriology 1998-09, Vol.180 (17), p.4487-4496
Hauptverfasser: Lubbers, M.W. (New Zealand Dairy Research Institute, Palmerston North, New Zealand.), Schofield, K, Waterfield, N.R, Polzin, K.M
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Sprache:eng
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Zusammenfassung:A detailed transcription map of the prolate-headed lactococcal phage c2 has been constructed. Transcription of about one-third of the genome, encoding 22 open reading frames, began within the first 2 min of infection and produced at least 12 overlapping transcripts that persisted until lysis occurred at 30 min after initiation of infection. The remaining two-thirds of the genome, encoding 17 open reading frames, was divergently transcribed, beginning between 4 and 6 min after initiation of infection, and resulted in at least 18 overlapping transcripts that persisted until lysis. Five very strong, simultaneously active, and probably unregulated early promoters and a single positively regulated late promoter were identified. The late promoter had an extended -10 sequence, had a significant basal level of activity in the uninduced slate, and was induced to high activity by a phage gene product. The complex overlapping pattern of transcripts resulted from the action of the multiple early promoters, inefficient termination of transcription, and (possibly) processing of a late precursor transcript(s). Phage proteins were not required for these processes, and the host RNA polymerase was probably used for both early and late transcription
ISSN:0021-9193
1098-5530
1067-8832
DOI:10.1128/JB.180.17.4487-4496.1998