Toxic effects of Cobalt II Chloride on tissue histopathology and serum proteins in Capoeta capoeta capoeta (Guldenstaedt 1772)

In this study, the effects of Cobalt (II) chloride (CoCI2) on Capoeta capoeta capoeta (Guldenstaedt 1772) were investigated by electrophoretic and histopathological methods. The fish from Kars Creek were placed in 500 liters tanks and they made to adapt into the medium for 15 days. Later, they were divided into 3 groups. The fish in the 1st group were held in normal water, 2nd and 3rd groups were held in the water containing 1 mg/L and 2 mg/L CoCl2, respectively for 10 days. At the end of this period, blood and tissue samples were taken from the fish for electrophoresis and histopathological examinations. Serum samples obtained were run in Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Tissue samples were fixed in %10 formaldehyde solution. Paraffin blocks were prepared by routine histological methods and slices 4-5 μ thickness were performed. The slices obtained were dyed with hematoxylen and eosin dying method and examined under light microscope. Thinnings in various protein types were observed in experimental groups in comparison to the control group in the electrophoregram. These thinnings were more in the group that 1 mg/L CoCl2 applied. In addition, formation of a 32.4 kD new protein band was observed in the group that 1 mg/L was applied; and 33.3 kD, 30.6 kD, and 28.2 kD new protein bands were observed in the group that 2 mg/L CoCl2 applied. In histopathological evaluations, an increase in the level of degeneration was observed in the livers and intestines tissues of the experimental fish groups in parallel to the increase of the dose. Due to the changes both in the serum protein expressions and in the tissues, we conclude that Cobalt can be dangerous at higher concentrations. Bu çalışmada, Capoeta capoeta capoeta (Guldenstaedt 1772) üzerine Kobalt (II) klorür'ün etkileri elektroforetik ve histopatolojik yöntemlerle araştırıldı. Kars Çayı'ndan yakalanan balıklar 500 litrelik tanklara konularak 15 gün süreyle ortama adaptasyonları sağlandı. Daha sonra 3 gruba ayrılarak I. gruptaki balıklar normal su ortamında, II. ve III. gruptaki balıklar ise sırasıyla 1 ve 2 mg/L CoCl2 içeren su ortamlarında 10 gün süreyle bekletildi. Bu süre sonunda elektroforetik ve histopatolojik çalışmalar için balıklardan kan ve doku örnekleri alındı. Elde edilen serum örnekleri Sodyum dodesil sülfat poliakrilamid jel elektroforezi (SDS-PAGE)'nde yürütüldü. Doku örnekleri ise %10'luk formaldehit solüsyonunda tespit edilerek rutin histolojik yöntemlerle