Rapid detection of aflatoxin by serological methods

Rapid detection of aflatoxin by serological methods

Antiserum was produced by using the following antigens: Aspergillus flavus (AFC), non aflatoxin producing, isolated in Thailand; A. flavus (AFB5), high aflatoxin producing, isolated in Thailand; A.parasiticus (AP827), high aflatoxin producing, isolated in U.S.A.; Aflatoxin B1 (AF). After collecting...

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Veröffentlicht in:Witthayāsān Kasētsārt. Sākhā thammācht 1991, Vol.25 (5)
Hauptverfasser: Pranee Hamelink, Chintana Chana, Chiradej Chamswarng (Kasetsart Univ., Bangkok (Thailand). Faculty of Agriculture. Dept. of Plant Pathology)
Format: Artikel
Sprache:eng
Schlagworte:
AFLATOXINAS
AFLATOXINE
ANIMAL DE LABORATOIRE
ANIMALES DE LABORATORIO
ASPERGILLUS FLAVUS
ASPERGILLUS PARASITICUS
ELISA
IMMUNSERUM
SUERO INMUNE
TECHNIQUE DE L'ISOLEMENT
TECNICAS DE AISLAMIENTO
TEST ELISA
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Zusammenfassung:Antiserum was produced by using the following antigens: Aspergillus flavus (AFC), non aflatoxin producing, isolated in Thailand; A. flavus (AFB5), high aflatoxin producing, isolated in Thailand; A.parasiticus (AP827), high aflatoxin producing, isolated in U.S.A.; Aflatoxin B1 (AF). After collecting normalserum, antigens were prepared and injected into 5 rabbits. Antisera were collected at weekly intervals 10-20 time. Only low titers of antisera could be detected by precipitation reaction on slides. The antisera were not sufficient for use in ELISA. Preliminary test using ALFA CHECK a product by UBE Industry Ltd, demonstrated clearly the high sensitivity of ELISA for Aflatoxin dectection. AFB 1 was detected at concentrations as low as 4 ppb in 20 minutes.
ISSN:0075-5192