Improvement of transformation efficiency of agrobacterium mediated gene transfer in tomato
Transformation/regeneration system was developed for agrobacterium tumefaciens mediated gene transfer in tomato. Transformation efficiency depended on variety and type of tissue of tomato. Cotyledon disc transformation was superior to leaf disc transformation. Transformants derived from cotyledon di...
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| Veröffentlicht in: | Witthayāsān Kasētsārt. Sākhā thammācht 1991, Vol.25 (5) |
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| container_title | Witthayāsān Kasētsārt. Sākhā thammācht |
| container_volume | 25 |
| creator | Supat Attathom Petcharat Siriwong Wichai Kositratana (Kasetsart Univ., Kamphaeng Saen Campus, Nakorn Pathom (Thailand). Plant Genetic Engineering Unit) |
| description | Transformation/regeneration system was developed for agrobacterium tumefaciens mediated gene transfer in tomato. Transformation efficiency depended on variety and type of tissue of tomato. Cotyledon disc transformation was superior to leaf disc transformation. Transformants derived from cotyledon disc transformation yielded 1-10 plantlets per tissue within 2-3 weeks. Transgenic tomato plants expressed kanamycin resistant gene and GUS (beta-glucuronidase) activity. |
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Cotyledon disc transformation was superior to leaf disc transformation. Transformants derived from cotyledon disc transformation yielded 1-10 plantlets per tissue within 2-3 weeks. Transgenic tomato plants expressed kanamycin resistant gene and GUS (beta-glucuronidase) activity.</abstract></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0075-5192 |
| ispartof | Witthayāsān Kasētsārt. Sākhā thammācht, 1991, Vol.25 (5) |
| issn | 0075-5192 |
| language | eng |
| recordid | cdi_fao_agris_TH9620554 |
| source | Alma/SFX Local Collection |
| subjects | AGROBACTERIUM TUMEFACIENS COTILEDONES COTYLEDON GENE GENES KANAMICINA KANAMYCINE LYCOPERSICON ESCULENTUM PLANTAS TRANSGENICAS PLANTE TRANSGENIQUE TEJIDOS VEGETALES TISSU VEGETAL TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE VARIEDADES VARIETE |
| title | Improvement of transformation efficiency of agrobacterium mediated gene transfer in tomato |
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