Improvement of transformation efficiency of agrobacterium mediated gene transfer in tomato

Improvement of transformation efficiency of agrobacterium mediated gene transfer in tomato

Transformation/regeneration system was developed for agrobacterium tumefaciens mediated gene transfer in tomato. Transformation efficiency depended on variety and type of tissue of tomato. Cotyledon disc transformation was superior to leaf disc transformation. Transformants derived from cotyledon di...

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Veröffentlicht in:Witthayāsān Kasētsārt. Sākhā thammācht 1991, Vol.25 (5)
Hauptverfasser: Supat Attathom, Petcharat Siriwong, Wichai Kositratana (Kasetsart Univ., Kamphaeng Saen Campus, Nakorn Pathom (Thailand). Plant Genetic Engineering Unit)
Format: Artikel
Sprache:eng
Schlagworte:
AGROBACTERIUM TUMEFACIENS
COTILEDONES
COTYLEDON
GENE
GENES
KANAMICINA
KANAMYCINE
LYCOPERSICON ESCULENTUM
PLANTAS TRANSGENICAS
PLANTE TRANSGENIQUE
TEJIDOS VEGETALES
TISSU VEGETAL
TRANSFORMACION GENETICA
TRANSFORMATION GENETIQUE
VARIEDADES
VARIETE
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Zusammenfassung:Transformation/regeneration system was developed for agrobacterium tumefaciens mediated gene transfer in tomato. Transformation efficiency depended on variety and type of tissue of tomato. Cotyledon disc transformation was superior to leaf disc transformation. Transformants derived from cotyledon disc transformation yielded 1-10 plantlets per tissue within 2-3 weeks. Transgenic tomato plants expressed kanamycin resistant gene and GUS (beta-glucuronidase) activity.
ISSN:0075-5192