Detection of sugarcane mosaic virus in sugarcane plantlets derived from meristem culture
Meristem culture of five cultivars of sugarcane, namely, Supan, Nigro, Macos, E-Heudang and CO were cultured on Marashige and Skoog media with 3 mg/l or 1 mg/l 2,4-D including 10 % (v/v) coconut water. The plantlet obtained were detected for the presence of sugarcane mosaic virus by using the enzyme...
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| Veröffentlicht in: | Witthayāsān Kasētsārt. Sākhā thammācht 1991, Vol.25 (5) |
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| container_title | Witthayāsān Kasētsārt. Sākhā thammācht |
| container_volume | 25 |
| creator | Rongrong Visessuwan (Kasetsart Univ. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Central Laboratory and Greenhouse Complex. Plant tissue culture Unit) Pissawan Chiemsombat Sontichai Chanprame |
| description | Meristem culture of five cultivars of sugarcane, namely, Supan, Nigro, Macos, E-Heudang and CO were cultured on Marashige and Skoog media with 3 mg/l or 1 mg/l 2,4-D including 10 % (v/v) coconut water. The plantlet obtained were detected for the presence of sugarcane mosaic virus by using the enzyme-linked immunosorbent assay (ELISA), with alkaline phosphatase and p-nitrophenyl reaction system. Results were evaluated by automatic reading machine and percentage of virus-free stock were determined. Sugarcane plantlets derived from meristem culture provided upto 80-100 % virus-free plants which could be mass propagated for furthur plantation in the field. |
| format | Article |
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Plant tissue culture Unit) ; Pissawan Chiemsombat ; Sontichai Chanprame</creator><creatorcontrib>Rongrong Visessuwan (Kasetsart Univ. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Central Laboratory and Greenhouse Complex. Plant tissue culture Unit) ; Pissawan Chiemsombat ; Sontichai Chanprame</creatorcontrib><description>Meristem culture of five cultivars of sugarcane, namely, Supan, Nigro, Macos, E-Heudang and CO were cultured on Marashige and Skoog media with 3 mg/l or 1 mg/l 2,4-D including 10 % (v/v) coconut water. The plantlet obtained were detected for the presence of sugarcane mosaic virus by using the enzyme-linked immunosorbent assay (ELISA), with alkaline phosphatase and p-nitrophenyl reaction system. Results were evaluated by automatic reading machine and percentage of virus-free stock were determined. Sugarcane plantlets derived from meristem culture provided upto 80-100 % virus-free plants which could be mass propagated for furthur plantation in the field.</description><identifier>ISSN: 0075-5192</identifier><language>eng</language><subject>2,4-D ; CULTIVO DE MERISTEMAS ; CULTURE DE MERISTEME ; ELISA ; SACCHARUM OFFICINARUM ; TECHNOLOGIE ; TECNOLOGIA ; TEST ELISA ; VIRUS DE LAS PLANTAS ; VIRUS DES VEGETAUX</subject><ispartof>Witthayāsān Kasētsārt. Sākhā thammācht, 1991, Vol.25 (5)</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Rongrong Visessuwan (Kasetsart Univ. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Central Laboratory and Greenhouse Complex. 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Sugarcane plantlets derived from meristem culture provided upto 80-100 % virus-free plants which could be mass propagated for furthur plantation in the field.</description><subject>2,4-D</subject><subject>CULTIVO DE MERISTEMAS</subject><subject>CULTURE DE MERISTEME</subject><subject>ELISA</subject><subject>SACCHARUM OFFICINARUM</subject><subject>TECHNOLOGIE</subject><subject>TECNOLOGIA</subject><subject>TEST ELISA</subject><subject>VIRUS DE LAS PLANTAS</subject><subject>VIRUS DES VEGETAUX</subject><issn>0075-5192</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><recordid>eNp9iksOgjAURTvQRKJswNHbAEnBFGTsJyyAgTPSwCup6cf0taxfTBw48k5OTs7dsIzzRhSibKsdy4me_LOG12eesccVI45RewdeAaVZhlE6BOtJ6hEWHRKBdj_lZaSLBiPBhEEvOIEK3oJdhSJaGJOJKeCBbZU0hPmXe3a83_pLVyjpBzmv56Hv2rriQpSnv_ENRoU7eA</recordid><startdate>1991</startdate><enddate>1991</enddate><creator>Rongrong Visessuwan (Kasetsart Univ. 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The plantlet obtained were detected for the presence of sugarcane mosaic virus by using the enzyme-linked immunosorbent assay (ELISA), with alkaline phosphatase and p-nitrophenyl reaction system. Results were evaluated by automatic reading machine and percentage of virus-free stock were determined. Sugarcane plantlets derived from meristem culture provided upto 80-100 % virus-free plants which could be mass propagated for furthur plantation in the field.</abstract></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0075-5192 |
| ispartof | Witthayāsān Kasētsārt. Sākhā thammācht, 1991, Vol.25 (5) |
| issn | 0075-5192 |
| language | eng |
| recordid | cdi_fao_agris_TH9620551 |
| source | Alma/SFX Local Collection |
| subjects | 2,4-D CULTIVO DE MERISTEMAS CULTURE DE MERISTEME ELISA SACCHARUM OFFICINARUM TECHNOLOGIE TECNOLOGIA TEST ELISA VIRUS DE LAS PLANTAS VIRUS DES VEGETAUX |
| title | Detection of sugarcane mosaic virus in sugarcane plantlets derived from meristem culture |
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