Detection of sugarcane mosaic virus in sugarcane plantlets derived from meristem culture

Meristem culture of five cultivars of sugarcane, namely, Supan, Nigro, Macos, E-Heudang and CO were cultured on Marashige and Skoog media with 3 mg/l or 1 mg/l 2,4-D including 10 % (v/v) coconut water. The plantlet obtained were detected for the presence of sugarcane mosaic virus by using the enzyme...

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Veröffentlicht in:	Witthayāsān Kasētsārt. Sākhā thammācht 1991, Vol.25 (5)
Hauptverfasser:	Rongrong Visessuwan (Kasetsart Univ. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Central Laboratory and Greenhouse Complex. Plant tissue culture Unit), Pissawan Chiemsombat, Sontichai Chanprame
Format:	Artikel
Sprache:	eng
Schlagworte:	2,4-D CULTIVO DE MERISTEMAS CULTURE DE MERISTEME ELISA SACCHARUM OFFICINARUM TECHNOLOGIE TECNOLOGIA TEST ELISA VIRUS DE LAS PLANTAS VIRUS DES VEGETAUX
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Beschreibung
Zusammenfassung:	Meristem culture of five cultivars of sugarcane, namely, Supan, Nigro, Macos, E-Heudang and CO were cultured on Marashige and Skoog media with 3 mg/l or 1 mg/l 2,4-D including 10 % (v/v) coconut water. The plantlet obtained were detected for the presence of sugarcane mosaic virus by using the enzyme-linked immunosorbent assay (ELISA), with alkaline phosphatase and p-nitrophenyl reaction system. Results were evaluated by automatic reading machine and percentage of virus-free stock were determined. Sugarcane plantlets derived from meristem culture provided upto 80-100 % virus-free plants which could be mass propagated for furthur plantation in the field.
ISSN:	0075-5192