Production of virus-free sugarcane by tissue culture

Production of virus-free sugarcane by tissue culture

Sugarcane plants of the three commercially grown varieties namely F140, Q83 and Supan I were produced free from sugarcane mosaic virus by the apical meristem culture. Shoot tips, 0.2-0.5 mm. in size, were cultured on MS medium supplemented with 3 mg/L 2,4-D and 15 % (v/v) coconut water. Yellowish-wh...

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Veröffentlicht in:Witthayāsān Kasētsārt. Sākhā thammācht 1988-10, Vol.22 (5)
Hauptverfasser: Rongrong Visessuwan, Wichai Korpraditskul, Supat Attathom (Kasetsart Univ., Kamphaengsaen Campus, Nakorn Pathom (Thailand). The Central Laboratory and Greenhouse Complex)
Format: Artikel
Sprache:eng
Schlagworte:
CULTIVO DE MERISTEMAS
CULTURE DE MERISTEME
PLANTAS LIBRES DE VIRUS
PLANTE INDEMNE DE VIRUS
SACCHARUM
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Zusammenfassung:Sugarcane plants of the three commercially grown varieties namely F140, Q83 and Supan I were produced free from sugarcane mosaic virus by the apical meristem culture. Shoot tips, 0.2-0.5 mm. in size, were cultured on MS medium supplemented with 3 mg/L 2,4-D and 15 % (v/v) coconut water. Yellowish-white callus developed within 2 months. Plantlets were regenerated from calli cultured on MS medium with 1 mg/L 2,4-D and 15 % (v/v) coconut water. The suitable medium for root induction in all cultivars was the MS or MS with 1 mg/L IBA. Detection of sugarcane mosaic virus (SCMV) in plantlets or individual plants by ELISA showed negative result suggesting that they were virus free.
ISSN:0075-5192