Localization of acetylated tubulin-positive nerve fibres in the spleen of sheep

The distribution of acetylated tubulin in the sheep spleen was studied using an indirect immunohistochemical method. A strong positive reaction for acetylated tubulin was observed on the nerve trunks and fine nerve fibres localized in the spleen hilus, fine nerve fibres were seen in the fibrous capsule and trabeculae. Accompanying large and medium-size branches of the splenic artery, nerve fibres enter the splenic parenchyma and as such are distributed in the white pulp. Most of the spleen nerve fibres positive for acetylated tubulin were found in the perivascular area, particularly arteries, arterioles, and the central artery of the splenic follicle. A fine network of nerve fibres was observed in the red pulp. After using the immunohistochemical method and acetylated tubulun antibody (Sigma), the present study displays the localisation of nerve fibres commnon in mammals