Dynamic associations of transcription factors with the rat liver nuclear matrix are functionally related to differential alfa-2-macroglobulin gene expression

Participation of the nuclear matrix in regulation of alfa-2-macroglobulin (alfa2M) gene transcription during rat liver development and the acute-phase (AP) response are examined. DNA affinity chromatography of fetal and adult liver internal nuclear matrix proteins under basal and AP conditions with...

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Veröffentlicht in:	Archives of biological sciences 2008, Vol.60 (3)
Hauptverfasser:	Dinic, S, Mihailovic, M, Ivanovic-Matic, S, Uskokovic, A, Grdovic, N, Vidakovic, M, Poznanovic, G., Institute for Biological Research Sinisa Stankovic, Belgrade (Serbia). Department of Molecular Biology
Format:	Artikel
Sprache:	eng
Schlagworte:	FACTEUR DE TRANSCRIPTION FACTORES DE TRANSCRIPCION FOIE GENE GENES HIGADO http://www.fao.org/aos/agrovoc#c_3214 http://www.fao.org/aos/agrovoc#c_35345 http://www.fao.org/aos/agrovoc#c_4395 http://www.fao.org/aos/agrovoc#c_6464 LIVER RAT RATA RATS TRANSCRIPTION FACTORS
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Zusammenfassung:	Participation of the nuclear matrix in regulation of alfa-2-macroglobulin (alfa2M) gene transcription during rat liver development and the acute-phase (AP) response are examined. DNA affinity chromatography of fetal and adult liver internal nuclear matrix proteins under basal and AP conditions with the alfa2M gene promotor (-852/+12) and immunoblot analysis revealed diverse patterns of association of transcription factors with the nuclear matrix. HNF-6, C/EBPalfa, and STAT5b were involved in basal and C/EBPbeta, STAT1, and STAT3 in AP-stimulated alfa2M expression. These findings support the assuption that transcription factor-nuclear matrix interactions serve to channel gene regulatory proteins to DNA sequences.
ISSN:	0354-4664