Effects of kanamycin on growth and development of Arabidopsis thaliana seedling, cotyledon and leaf

In this research, growth and development of Arabidopsis thaliana seedling cotyledon and leaf were distinctly influenced by kanamycin. Contrasted against the control, cotyledon on MS with kanamycin was very small and took on etiolation, etiolation of cotyledon was serious and some even died as cultured for 10 d. Along with culture time increasing, cells in the epidermis tissue of cotyledon on MS with kanamycin were irregularly arranged, the intercellular space in the mesophyll tissue was large, and ability of cell division in the meristematic zone of shoot tip gradually weakened. In addition, it was detected that effects of kanamycin on seedling cotyledon and shoot tip exhibited reversibility to a certain extent. Accordingly, it is guessed that kanamycin may affect growth of Arabidopsis thaliana seedling cotyledon and leaf by restraining protein synthesis, and then influence growth of Arabidopsis thaliana seedling.