Effect of glucose supplementation in skim milk diluter on motility, plasma membrane and acrosomal integrity of nili-ravi buffalo bull spermatozoa stored at 5 degree C

This experiment was designed to evaluate the effect of glucose supplementation in skim milk diluter on motility, plasma membrane and acrosomal integrity of Nili-Ravi buffalo bull spermatozoa stored at 5 degree C. Semen from three buffalo bulls was collected and diluted at 37 degree C with a final concentration of 10 x 10(6) motile spermatozoa per ml in skim milk diluter containing 5, 10 and 30mM glucose or without glucose (control). The diluted semen was cooled to 5 degree C in 2 hours and stored at 5 degree C for five days. Motility and plasma membrane integrity of buffalo bull spermatozoa was similar (P greater than 0.05) in all four experimental diluters at 1st day of storage. However, sperm motility and plasma membrane integrity on 3rd and 5th day of storage was significantly (P less than 0.05) higher in diluter containing glucose (5, 10 or 30mM) as compared to control. Sperm acrosomal integrity remained similar (P greater than 0.05) in all experimental diluters for five days of storage at 5 degree C. No differences were found among experimental diluters supplemented with 5, 10 and 30mM glucose (P less than 0.05), therefore, 5mM glucose supplementation is recommended. In conclusion, glucose supplementation improved the preservability of Nili-Ravi buffalo bull spermatozoa in skim milk diluter at 5 degree C.