In vitro regeneration of plantlets from seedlings explants of guava (Psidium guajava L.) cv. safeda
Regeneration of plantlets from In vitro germinated seedling explants was carried out at NIFA, Peshawar. Multiple shoots were generated from In vitro germinated (50 days-old) seedlings of Psidium guajava L. cv. Safeda. Modified Murashige & Skoog (MMS) medium supplemented with 6-benzylaminopurine...
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| Veröffentlicht in: | Pakistan journal of botany 2008-06, Vol.40 (3), p.1195-1200 |
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| container_title | Pakistan journal of botany |
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| creator | Shah, S.T. (Nuclear Inst. for Food and Agriculture, Peshawar (Pakistan)) Zamir, R. (Nuclear Inst. for Food and Agriculture, Peshawar (Pakistan)) Ahmad, J. (Peshawar Univ. (Pakistan). Centre of Biotechnology) Ali, H. (Karachi Univ. (Pakistan). Dept. of Botany) Lutfullah, G |
| description | Regeneration of plantlets from In vitro germinated seedling explants was carried out at NIFA, Peshawar. Multiple shoots were generated from In vitro germinated (50 days-old) seedlings of Psidium guajava L. cv. Safeda. Modified Murashige & Skoog (MMS) medium supplemented with 6-benzylaminopurine (BAP), Zeatin and Gibberellic acid (GA3), Zeatin 1.0 mg/L combined with GA3 0.5 mg/L gave the highest response (47.6%) with the regeneration of (3.2) shoots per original explants. Each shoot gave rise to about 4.4 shoots per explant upon sub culture on MMS supplemented with BAP 1.0 mg/L combined with Kinetin 0.5 mg/L. The micro shoots were successfully rooted on 1/2 strength MMS medium supplemented with IBA 1.5 mg/L and NAA 0.5 mg/L, where 85% rooted plantlets were obtained. The In vitro plantlets after rooting were acclimatized in Jiffy pots and established in the soil. |
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(Nuclear Inst. for Food and Agriculture, Peshawar (Pakistan)) ; Zamir, R. (Nuclear Inst. for Food and Agriculture, Peshawar (Pakistan)) ; Ahmad, J. (Peshawar Univ. (Pakistan). Centre of Biotechnology) ; Ali, H. (Karachi Univ. (Pakistan). Dept. of Botany) ; Lutfullah, G</creator><creatorcontrib>Shah, S.T. (Nuclear Inst. for Food and Agriculture, Peshawar (Pakistan)) ; Zamir, R. (Nuclear Inst. for Food and Agriculture, Peshawar (Pakistan)) ; Ahmad, J. (Peshawar Univ. (Pakistan). Centre of Biotechnology) ; Ali, H. (Karachi Univ. (Pakistan). Dept. of Botany) ; Lutfullah, G</creatorcontrib><description>Regeneration of plantlets from In vitro germinated seedling explants was carried out at NIFA, Peshawar. Multiple shoots were generated from In vitro germinated (50 days-old) seedlings of Psidium guajava L. cv. Safeda. Modified Murashige & Skoog (MMS) medium supplemented with 6-benzylaminopurine (BAP), Zeatin and Gibberellic acid (GA3), Zeatin 1.0 mg/L combined with GA3 0.5 mg/L gave the highest response (47.6%) with the regeneration of (3.2) shoots per original explants. Each shoot gave rise to about 4.4 shoots per explant upon sub culture on MMS supplemented with BAP 1.0 mg/L combined with Kinetin 0.5 mg/L. The micro shoots were successfully rooted on 1/2 strength MMS medium supplemented with IBA 1.5 mg/L and NAA 0.5 mg/L, where 85% rooted plantlets were obtained. The In vitro plantlets after rooting were acclimatized in Jiffy pots and established in the soil.</description><identifier>ISSN: 0556-3321</identifier><language>eng</language><subject>APPLICATION RATES ; CULTURE MEDIA ; EXPLANTS ; GIBBERELLIC ACID ; IN VITRO REGENERATION ; KINETIN ; PSIDIUM GUAJAVA ; ROOTING ; SEEDLINGS ; VARIETIES ; ZEATIN</subject><ispartof>Pakistan journal of botany, 2008-06, Vol.40 (3), p.1195-1200</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids></links><search><creatorcontrib>Shah, S.T. (Nuclear Inst. for Food and Agriculture, Peshawar (Pakistan))</creatorcontrib><creatorcontrib>Zamir, R. (Nuclear Inst. for Food and Agriculture, Peshawar (Pakistan))</creatorcontrib><creatorcontrib>Ahmad, J. (Peshawar Univ. (Pakistan). Centre of Biotechnology)</creatorcontrib><creatorcontrib>Ali, H. (Karachi Univ. (Pakistan). Dept. of Botany)</creatorcontrib><creatorcontrib>Lutfullah, G</creatorcontrib><title>In vitro regeneration of plantlets from seedlings explants of guava (Psidium guajava L.) cv. safeda</title><title>Pakistan journal of botany</title><description>Regeneration of plantlets from In vitro germinated seedling explants was carried out at NIFA, Peshawar. Multiple shoots were generated from In vitro germinated (50 days-old) seedlings of Psidium guajava L. cv. Safeda. Modified Murashige & Skoog (MMS) medium supplemented with 6-benzylaminopurine (BAP), Zeatin and Gibberellic acid (GA3), Zeatin 1.0 mg/L combined with GA3 0.5 mg/L gave the highest response (47.6%) with the regeneration of (3.2) shoots per original explants. Each shoot gave rise to about 4.4 shoots per explant upon sub culture on MMS supplemented with BAP 1.0 mg/L combined with Kinetin 0.5 mg/L. The micro shoots were successfully rooted on 1/2 strength MMS medium supplemented with IBA 1.5 mg/L and NAA 0.5 mg/L, where 85% rooted plantlets were obtained. The In vitro plantlets after rooting were acclimatized in Jiffy pots and established in the soil.</description><subject>APPLICATION RATES</subject><subject>CULTURE MEDIA</subject><subject>EXPLANTS</subject><subject>GIBBERELLIC ACID</subject><subject>IN VITRO REGENERATION</subject><subject>KINETIN</subject><subject>PSIDIUM GUAJAVA</subject><subject>ROOTING</subject><subject>SEEDLINGS</subject><subject>VARIETIES</subject><subject>ZEATIN</subject><issn>0556-3321</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNotjLtOwzAUhj2ARCk8ApJHGFL5EtvJiCouFZHoAHN1Ep8TuUoTFLsRj08CTJ_-i74LthLG2ExrJa_YdYxHIawujF2xZtfzKaRx4CO22OMIKQw9H4h_ddCnDlPkNA4nHhF9F_o2cvz-neJyas8wAb_fx-DD-bTE41JUmwfeTBsegdDDDbsk6CLe_nPNPp-fPravWfX-sts-VhlJ51IG0ktLWHvhNWHZeCFVWRvdUC7yQjW1sgUQld6ZGaisNW4ufS61s1BbvWZ3f16C4QDtGOJh_6aEFGI25U7_ADQyTkw</recordid><startdate>200806</startdate><enddate>200806</enddate><creator>Shah, S.T. 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Centre of Biotechnology)</creatorcontrib><creatorcontrib>Ali, H. (Karachi Univ. (Pakistan). Dept. of Botany)</creatorcontrib><creatorcontrib>Lutfullah, G</creatorcontrib><collection>AGRIS</collection><jtitle>Pakistan journal of botany</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shah, S.T. (Nuclear Inst. for Food and Agriculture, Peshawar (Pakistan))</au><au>Zamir, R. (Nuclear Inst. for Food and Agriculture, Peshawar (Pakistan))</au><au>Ahmad, J. (Peshawar Univ. (Pakistan). Centre of Biotechnology)</au><au>Ali, H. (Karachi Univ. (Pakistan). 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Each shoot gave rise to about 4.4 shoots per explant upon sub culture on MMS supplemented with BAP 1.0 mg/L combined with Kinetin 0.5 mg/L. The micro shoots were successfully rooted on 1/2 strength MMS medium supplemented with IBA 1.5 mg/L and NAA 0.5 mg/L, where 85% rooted plantlets were obtained. The In vitro plantlets after rooting were acclimatized in Jiffy pots and established in the soil.</abstract><tpages>6</tpages></addata></record> |
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| ispartof | Pakistan journal of botany, 2008-06, Vol.40 (3), p.1195-1200 |
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| language | eng |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | APPLICATION RATES CULTURE MEDIA EXPLANTS GIBBERELLIC ACID IN VITRO REGENERATION KINETIN PSIDIUM GUAJAVA ROOTING SEEDLINGS VARIETIES ZEATIN |
| title | In vitro regeneration of plantlets from seedlings explants of guava (Psidium guajava L.) cv. safeda |
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