In vitro regeneration of plantlets from seedlings explants of guava (Psidium guajava L.) cv. safeda

Regeneration of plantlets from In vitro germinated seedling explants was carried out at NIFA, Peshawar. Multiple shoots were generated from In vitro germinated (50 days-old) seedlings of Psidium guajava L. cv. Safeda. Modified Murashige & Skoog (MMS) medium supplemented with 6-benzylaminopurine (BAP), Zeatin and Gibberellic acid (GA3), Zeatin 1.0 mg/L combined with GA3 0.5 mg/L gave the highest response (47.6%) with the regeneration of (3.2) shoots per original explants. Each shoot gave rise to about 4.4 shoots per explant upon sub culture on MMS supplemented with BAP 1.0 mg/L combined with Kinetin 0.5 mg/L. The micro shoots were successfully rooted on 1/2 strength MMS medium supplemented with IBA 1.5 mg/L and NAA 0.5 mg/L, where 85% rooted plantlets were obtained. The In vitro plantlets after rooting were acclimatized in Jiffy pots and established in the soil.