Plant regeneration from hypocotyl culture of Peganum harmala

Plant regeneration from hypocotyl culture of Peganum harmala

Conditions for plant regeneration from excised hypocotyl of Peganumharmala were studied. Complete plants were regenerated directly fromhypocotyl segments on MS medium supplemented with BAP, Kinetin and NAA.Multiple shoots were regenerated from 87% of explants. Addition of thiaminehydrochloride to th...

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Veröffentlicht in:Pakistan journal of botany 2002-09, Vol.34 (3)
Hauptverfasser: Ehsanpour, A.A, Sa Adat, E.,University of Isfahan (Iran). Dept. ofBiology
Format: Artikel
Sprache:eng
Schlagworte:
CULTURE
DRUG PLANTS
HYPOCOTYLS
TISSUE CULTURE
ZYGOPHYLLACEAE
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Zusammenfassung:Conditions for plant regeneration from excised hypocotyl of Peganumharmala were studied. Complete plants were regenerated directly fromhypocotyl segments on MS medium supplemented with BAP, Kinetin and NAA.Multiple shoots were regenerated from 87% of explants. Addition of thiaminehydrochloride to the culture medium increased the number of explants whichregenerated and the number of shoots per explants. Regenerated shoots wererooted on MS medium supplemented with or without NAA. The morphology ofwhole plants was similar to the original plants.
ISSN:0556-3321