Optimization of Conditions for the Production and Properties of Alginate-degrading Crude Enzyme from Shewanella oneidensis PKA 1008

Optimization of Conditions for the Production and Properties of Alginate-degrading Crude Enzyme from Shewanella oneidensis PKA 1008

An alginate-degrading bacterium, identified as Shewanella oneidensis PKA 1008 by 16S ribosomal RNA sequence analysis, was isolated from the green alga Ulva pertusa. Optimal conditions for the alginate- degrading ability of its crude enzyme were then determined. The optimal culture conditions for the...

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Veröffentlicht in:Korean Journal of Microbiology and Biotechnology 2013-09, Vol.41 (3)
Hauptverfasser: Sunwoo, C., Pukyong National University, Busan, Republic of Korea, Kim, K.B.W.R., Pukyong National University, Busan, Republic of Korea, Kim, D.H., Pukyong National University, Busan, Republic of Korea, Jung, S.A., Pukyong National University, Busan, Republic of Korea, Kim, H.J., Pukyong National University, Busan, Republic of Korea, Jeong, D.H., Pukyong National University, Busan, Republic of Korea, Jung, H.Y., Pukyong National University, Busan, Republic of Korea, Kang, B.K., Pukyong National University, Busan, Republic of Korea, Bark, S.W., Pukyong National University, Busan, Republic of Korea, Lim, S.M., Tongmyong University, Busan, Republic of Korea, Hong, Y.K., Tongmyong University, Busan, Republic of Korea, Ahn, D.H, Pukyong National University, Busan, Republic of Korea
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Alginate-degrading crude enzyme, Shewanella oneidensis PKA 1008
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Zusammenfassung:An alginate-degrading bacterium, identified as Shewanella oneidensis PKA 1008 by 16S ribosomal RNA sequence analysis, was isolated from the green alga Ulva pertusa. Optimal conditions for the alginate- degrading ability of its crude enzyme were then determined. The optimal culture conditions for the growth of S. oneidensis PKA 1008 were pH 9, 2% NaCl, 30℃, and 24 hours incubation time. The crude enzyme produced by S. oneidensis PKA 1008 showed the highest alginate-degrading activity at pH 9, 30℃ and produced 1.001 g of reducing sugar per liter in 3.5% (w/v) sodium alginate for 1 hour.
ISSN:1598-642X
DOI:10.4014/kjmb.1212.12002