Distribution of Ogura Cytoplasmic Male Sterility in Some Germplasms of Radish (Raphanus sativus)

A primer pair of the orf138 gene of Ogura cytoplasmic male sterility of radish, Raphanus sativus, was constructed according to the sequences of GenBank Accession No. AB055442. The male sterile plants appeared unexpectedly in the selfed generation of Korean wild radishes and a collected local cultivar was detected with these primers. All showed the specific band of Ogura CMS as expected. Thus it was applied moreover to a total of 171 entries consisting of 62 wild radishes collected from 3 main areas in Korea, 31 local cultivars kept in the seed bank of Rural Development Administration, 19 F₁ cultivars distributed by a seed company in Korea, and 59 oriental accessions introduced mainly from China and Russia. On the whole, 62 entries presented the specific band of orf138 gene in the PCR. In detail, no local cultivar presented it, while about 74% of 62 wild radishes showed it. Eleven out of 59 introductions and 5 of 19 Korean F₁ cultivars presented it.