Selection of nptII Transgenic Sweetpotato Plants Using G∧418 and Paromomycin
We have used two aminoglycosides, G∧418 and paromomycin, to develop a reliable selection system for nptII transgenic sweetpotato (Ipomoea batatas (L.) Lam.). Embryogenic calli derived from shoot apical meristems were bombarded with gold particles coated with pCAMBIA2301, which contained the nptII an...
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| Veröffentlicht in: | Journal of plant biology = Singmul Hakhoe chi 2007-04, Vol.50 (2) |
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| creator | Shin, Y.M. (Chonnam National University, Gwangju, Republic of Korea) Choe, G. (Chonnam National University, Gwangju, Republic of Korea) Shin, B.C. (Chonnam National University, Gwangju, Republic of Korea) Yi, G.B. (Chonnam National University, Gwangju, Republic of Korea) Yun, P.Y. (Jeju Hi-Tech Industry Development Institute, Jeju, Republic of Korea) Yang, K.Y. (Chonnam National University, Gwangju, Republic of Korea) Lee, J.S. (Mokpo Experiment Station, NICS, RDA, Mokpo, Republic of Korea) Kwak, S.S. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea) Kim, K.M. (Chonnam National University, Gwangju, Republic of Korea), E-mail: kmkimus@hanmail.net |
| description | We have used two aminoglycosides, G∧418 and paromomycin, to develop a reliable selection system for nptII transgenic sweetpotato (Ipomoea batatas (L.) Lam.). Embryogenic calli derived from shoot apical meristems were bombarded with gold particles coated with pCAMBIA2301, which contained the nptII and gusA genes. When compared on a kill curve that was based on calli proliferation and cell viability, G∧418-selection proved to be more efficient and had fewer escapes than kanamycin. These bombarded explants were then selected on G∧418-containing media. |
| format | Article |
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(Chonnam National University, Gwangju, Republic of Korea) ; Lee, J.S. (Mokpo Experiment Station, NICS, RDA, Mokpo, Republic of Korea) ; Kwak, S.S. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea) ; Kim, K.M. (Chonnam National University, Gwangju, Republic of Korea), E-mail: kmkimus@hanmail.net</creatorcontrib><description>We have used two aminoglycosides, G∧418 and paromomycin, to develop a reliable selection system for nptII transgenic sweetpotato (Ipomoea batatas (L.) Lam.). Embryogenic calli derived from shoot apical meristems were bombarded with gold particles coated with pCAMBIA2301, which contained the nptII and gusA genes. When compared on a kill curve that was based on calli proliferation and cell viability, G∧418-selection proved to be more efficient and had fewer escapes than kanamycin. 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(Chonnam National University, Gwangju, Republic of Korea) ; Shin, B.C. (Chonnam National University, Gwangju, Republic of Korea) ; Yi, G.B. (Chonnam National University, Gwangju, Republic of Korea) ; Yun, P.Y. (Jeju Hi-Tech Industry Development Institute, Jeju, Republic of Korea) ; Yang, K.Y. (Chonnam National University, Gwangju, Republic of Korea) ; Lee, J.S. (Mokpo Experiment Station, NICS, RDA, Mokpo, Republic of Korea) ; Kwak, S.S. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea) ; Kim, K.M. 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(Chonnam National University, Gwangju, Republic of Korea)</au><au>Shin, B.C. (Chonnam National University, Gwangju, Republic of Korea)</au><au>Yi, G.B. (Chonnam National University, Gwangju, Republic of Korea)</au><au>Yun, P.Y. (Jeju Hi-Tech Industry Development Institute, Jeju, Republic of Korea)</au><au>Yang, K.Y. (Chonnam National University, Gwangju, Republic of Korea)</au><au>Lee, J.S. (Mokpo Experiment Station, NICS, RDA, Mokpo, Republic of Korea)</au><au>Kwak, S.S. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea)</au><au>Kim, K.M. 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These bombarded explants were then selected on G∧418-containing media.</abstract></addata></record> |
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| language | eng |
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| subjects | BIOLISTICA BIOLISTICS BIOLISTIQUE GENETIC ENGINEERING GENIE GENETIQUE GUS INGENIERIA GENETICA Ipomoea batatas (L.) Lam shoot apical meristem |
| title | Selection of nptII Transgenic Sweetpotato Plants Using G∧418 and Paromomycin |
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