Selection of nptII Transgenic Sweetpotato Plants Using G∧418 and Paromomycin

Selection of nptII Transgenic Sweetpotato Plants Using G∧418 and Paromomycin

We have used two aminoglycosides, G∧418 and paromomycin, to develop a reliable selection system for nptII transgenic sweetpotato (Ipomoea batatas (L.) Lam.). Embryogenic calli derived from shoot apical meristems were bombarded with gold particles coated with pCAMBIA2301, which contained the nptII an...

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Veröffentlicht in:Journal of plant biology = Singmul Hakhoe chi 2007-04, Vol.50 (2)
Hauptverfasser: Shin, Y.M. (Chonnam National University, Gwangju, Republic of Korea), Choe, G. (Chonnam National University, Gwangju, Republic of Korea), Shin, B.C. (Chonnam National University, Gwangju, Republic of Korea), Yi, G.B. (Chonnam National University, Gwangju, Republic of Korea), Yun, P.Y. (Jeju Hi-Tech Industry Development Institute, Jeju, Republic of Korea), Yang, K.Y. (Chonnam National University, Gwangju, Republic of Korea), Lee, J.S. (Mokpo Experiment Station, NICS, RDA, Mokpo, Republic of Korea), Kwak, S.S. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea), Kim, K.M. (Chonnam National University, Gwangju, Republic of Korea), E-mail: kmkimus@hanmail.net
Format: Artikel
Sprache:eng
Schlagworte:
BIOLISTICA
BIOLISTICS
BIOLISTIQUE
GENETIC ENGINEERING
GENIE GENETIQUE
GUS
INGENIERIA GENETICA
Ipomoea batatas (L.) Lam
shoot apical meristem
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Zusammenfassung:We have used two aminoglycosides, G∧418 and paromomycin, to develop a reliable selection system for nptII transgenic sweetpotato (Ipomoea batatas (L.) Lam.). Embryogenic calli derived from shoot apical meristems were bombarded with gold particles coated with pCAMBIA2301, which contained the nptII and gusA genes. When compared on a kill curve that was based on calli proliferation and cell viability, G∧418-selection proved to be more efficient and had fewer escapes than kanamycin. These bombarded explants were then selected on G∧418-containing media.
ISSN:1226-9239
1867-0725