Direct and Indirect Regeneration of Plants from Internodal and Leaf Explants of Hypericum bupleuroides Gris

Species of the genus Hypericum are of considerable interest worldwide because of their medicinal properties. In-vitro culture is a useful tool for both multiplication of the genus and studying its economically important secondary metabolites. Here, we present an effective in-vitro propagation method for H. bupleuroides. Leaf and internodal explants excised from 9-week-old, in vitro-germinated seedlings were cultured on a Murashige and Skoog (MS) medium supplemented with benzyladenine (BA; 1.0 or 0.1 mg L-¹) and 2,4-dichlorophenoxyacetic acid (2,4-D; 1.0 or 0.1 mg L-¹).