Evaluation of Mytagenicity with Gamgung-tang Using Host-Mediated Assay

Mutagenicity of Gamgung-tang (GGT) was tested using in vitro S-9 mixture and in vivo host-mediated assay with Salmonella typhimurium. In the previous reports, GGT was tested for the safety using Ames(-S-9), Bacillus subtilis Rec, and umu gene expression mutagenicity tests. Mutagenic activity in any assays we tested was not found. In this report, we further investigated safety of GGT after metabolic activation in vivo. Ames test with S-9 mixture and host-mediated assay with Salmonella typhimurium TA98 were used to identify metagenic property of GGT.